Restriction Endonucleases
Restriction enzymes are highly specific nucleases which occur ubiquitously among prokaryotic organisms, where they serve to protect bacterial cells against foreign DNA. Many different types of restriction enzymes are known, among them multi-subunit enzyme
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Series Editor H. J. Gross
Springer-Verlag Berlin Heide1berg GmbH
Alfred M. Pingoud (Ed.)
Restriction Endonucleases
With 100 Figures, 27 of Them in Color
Springer
Professor Dr. ALFRED M. PINGOUD Institute for Biochemistry Justus-Liebig-University Heinrich-Buff-Ring 58 35392 Giessen Germany
ISSN 0933-1891 ISBN 978-3-642-62324-0 Library of Congress Cataloging-in-Publication Data Restriction endonudeases / Alfred M. Pingoud. p. cm. -- (Nucleic acids and molecular biology) Includes bibliographical references and index. ISBN 978-3-642-62324-0 ISBN 978-3-642-18851-0 (eBook) DOI 10.1007/978-3-642-18851-0 1. Restriction enzymes, DNA. 2. Endonucleases. 3. Gene amplification. 1. Pingoud,A. (Alfred) Il. Series. QP609.R44R4732003 572'.785--dc22 2003064952
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Preface
Approximately 50 years ago the phenomenon of restriction was first described. This led later to the discovery of restriction (and modification) enzymes by Werner Arber, Daniel Nathans and Hamilton Smith who received the Nobel Prize in Physiology or Medicine 1978"for the discovery of restriction enzymes and their application to problems of molecular genetics" . In retrospect, it is clear that the impact of this discovery was much greater: there would be no recombinant DNA revolution and no gene technology without restriction endonucleases. However,beyond their tremendous importance as tools for the analysis and recombination of DNA, restriction enzymes have provided outstanding model systems to study many aspects of proteinnucleic acid interactions, e.g., how proteins find their target sites within a great excess of non-specific sites, how short DNA sequences are recognized with such extreme accuracy, and how recogn ition is so efficiently coupled to catalysis. Restriction enzymes are usually part of restriction-modification (R-M) syst
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