RETRACTED ARTICLE: Changes in the electron paramagnetic resonance spectra of albumin-associated spin-labeled stea
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WORLD JOURNAL OF SURGICAL ONCOLOGY
RESEARCH
Open Access
Changes in the electron paramagnetic resonance spectra of albumin-associated spin-labeled stearic acid as a diagnostic parameter of colorectal cancer Zhongchao Liu, Wenyi Zhang, Saijun Fan, Liang Wang and Ling Jiao*
Abstract Background: With more than 940,000 new colorectal cancer cases worldwide each year, there is no better way for colorectal cancer routine screening. The aim of this study was to investigate whether the fatty acid binding to albumin is detectably and significantly altered in colorectal cancer patients when compared with healthy people, in order to find a better way for colorectal cancer diagnosis. Methods: One hundred and forty-one patients operatively treated for colorectal cancer were included in the examination, and 180 healthy people were also enrolled as controls. Commercial 16-doxyl stearic acid was used as spin probe. Serum albumin was analyzed by electron paramagnetic resonance (EPR) with spin probe. Discriminant analysis was carried out using the measured EPR spectra by SPSS 20.0. Results: Of the original grouped cases, 89.4% were correctly classified. Of the cross-validated grouped cases, 86.9% were correctly classified. Using Fisher linear discriminant analysis we were able to develop a mathematical model allowing for identification of colorectal cancer patients based on five values (both relative intensity and peak width) which are obtained from the EPR spectrum. Conclusions: Cancer-associated alterations to albumin can be assessed by spin-label EPR. The potential applications for this diagnostic technique are significant and represent a cost-effective means for screening patients with cancer. Spin probe for diagnosis of colorectal cancer might be a useful tool and further studies should take place in order to investigate all stages of colorectal cancer patients. Keywords: Colorectal cancer, Discriminant analysis, EPR/ESR, Spin probe
Background Colorectal cancer is the second most common cancer and the second most common cause of death by cancer [1]. The clinical stage of the disease at diagnosis often determines the prognosis and survival rate of a patient with colorectal cancer [2]. If the colorectal cancer patient could be diagnosed at an early stage, they will have a better treatment than if diagnosed at an advanced stage. However, insufficient evidence concerning prognostic and predictive value exists for other molecular factors such as thymidylate synthase, microsatellite instability (MSI), p-53 and K-ras. [3]. * Correspondence: [email protected] Department of Radiation Protection Center, Institute of Radiation Medicine, Chinese Academy of Medical Sciences&Peking Union Medical College, Tianjin Key Laboratory of Molecular Nuclear Medicine, 238 Baidi Road, Nankai District, Tianjin 300192, P.R. China
Human serum albumin (HSA) is the main component for transport of a variety of peptides and of waterinsoluble fatty acids (FA) in the serum [4]. Its remarkable ability for binding FA has motivated our group to use spin-labele
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