Reviews and Protocols in DT40 Research Subcellular Biochemistry
Research is fast paced and advances in RNA interference have recently opened up new opportunities for genetic experiments in human cell lines. However, the possibility to easily modify the genome still remains a powerful tool to investigate the function o
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SUBCELLULAR BIOCHEMISTRY SERIES EDITOR J. ROBIN HARRIS, University of Mainz, Germany
Reviews and Protocols in DT40 Research Subcellular Biochemistry Volume 40 Edited by
Jean-Marie Buerstedde GSF National Research Centre, Munich, Germany
and
Shunichi Takeda Kyoto University, Japan
This series is a continuation of the journal Sub-Cellular Biochemistry Volume 1 to 4 of which were published quarterly from 1972 to 1975 ISBN-10 1-4020-4895-5 (HB) ISBN-13 978-1-4020-4895-1 (HB) ISBN-10 1-4020-4896-3 (e-book) ISBN-13 978-1-4020-4896-8 (e-book)
© 2006 Springer All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Springer Science + Business Media, Inc., 233 Springer street, New York, NY 10013, USA), except for brief excerpts in connection with review or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks and similar terms, whether or not they are subject to proprietary rights. (BS/DH) 9 8
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CONTENTS
Foreword....................................................................................................... xi CHAPTER 1. DT40 GENE DISRUPTIONS: A HOW-TO FOR THE DESIGN AND THE CONSTRUCTION OF TARGETING VECTORS ...................................................................................................... 1 Hiroshi Arakawa and Jean-Marie Buerstedde CHAPTER 2. IMMUNOGLOBULIN GENE CONVERSION OR HYPERMUTATION: THAT’S THE QUESTION ............................... 11 Jean-Marie Buerstedde and Hiroshi Arakawa CHAPTER 3. GENOME RESOURCES FOR THE DT40 COMMUNITY ............................................................................................ 25 Randolph B. Caldwell and Andrzej M. Kierzek CHAPTER 4. CHROMOSOME ENGINEERING IN DT40 CELLS AND MAMMALIAN CENTROMERE FUNCTION................................ 39 William R.A. Brown, Margaret C.M. Smith, Felix Dafhnis-Calas, Sunir Malla and Zhengyao Xu CHAPTER 5. FUNCTION OF RECQ FAMILY HELICASE IN GENOME STABILITY ......................................................................... 49 Masayuki Seki, Shusuke Tada and Takemi Enomoto CHAPTER 6. GENETIC ANALYSIS OF APOPTOTIC EXECUTION .............................................................................................. 75 Sandrine Ruchaud, Kumiko Samejima, Damien Hudson, Scott H. Kaufmann and William C. Earnshaw CHAPTER 7. THE DT40 SYSTEM AS A TOOL FOR ANALYZING KINETOCHORE ASSEMBLY................................................................... 91 Masahiro Okada, Tetsuya Hori and Tatsuo Fukagawa CHAPTER 8. ANALYSING THE DNA DAMAGE AND REPLICATION CHECKPOINTS IN DT40 CELLS .......................107 Michael D. Rainey, George Zachos and David A.F. Gillespie
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Contents
CHAPTER 9. USING DT40 TO STUDY CLATHRIN FUNCTION....... 119 Frank R. Wettey and Antony P. J
