Rice-specific Argonaute 17 controls reproductive growth and yield-associated phenotypes
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Rice‑specific Argonaute 17 controls reproductive growth and yield‑associated phenotypes Kannan Pachamuthu1 · Chenna Swetha1 · Debjani Basu1 · Soumita Das1 · Indira Singh1 · Vivek Hari Sundar1 · T. N. Sujith1 · Padubidri V. Shivaprasad1 Received: 31 May 2020 / Accepted: 8 September 2020 © Springer Nature B.V. 2020
Abstract Key Message This manuscript describes the functions of an Argonaute protein named AGO17 in rice. AGO17 is required for the development of rice reproductive tissues. Abstract Argonaute (AGO) proteins are a well-conserved multigene family of regulators mediating gene silencing across eukaryotes. Monocot plants have additional members of AGO, the functions of which are poorly understood. Among the non-dicot AGO1 clade members in monocots, AGO17 expresses highly in reproductive tissues. Here we show that overexpression of Oryza sativa indica AGO17 in rice resulted in robust growth and increased yield, whereas its silencing resulted in reduced panicle length, less fertility, and poor growth. Small (s)RNA transcriptome analysis revealed misregulation of several miRNAs and other categories of sRNAs in silenced and overexpression lines, in agreement with its likely competition with other AGO1 clade members. Targets of differentially expressed miRNAs included previously unreported target RNAs coding for proteins involved in development, phase transition, and transport. Our results indicate a distinctive role for OsAGO17 in rice reproductive development that could be harnessed to improve yield. Keywords Rice · Argonaute · Development · miRNA · Gene silencing · siRNA
Introduction RNA silencing is a gene regulatory mechanism that is evolutionarily conserved across eukaryotes (Baulcombe 2004). This pathway is mediated by a set of small noncoding (s) RNAs of approximately 20 to 24 nucleotides (nt) in length. sRNAs are produced from long precursor RNAs of varied Accession numbers Small RNA sequencing data for rice lines are available in Gene Expression Omnibus (GEO) series GSE144110. RNA-Seq and degradome datasets mentioned in the manuscript can be accessed using GSE111438 and GSE118702, respectively. Accession numbers of genes mentioned in the manuscript can be found in Supplementary Table S1. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11103-020-01071-2) contains supplementary material, which is available to authorized users. * Padubidri V. Shivaprasad [email protected] 1
National Centre for Biological Sciences, Tata Institute of Fundamental Research, GKVK Campus, Bangalore 560065, India
length, source, and structure by the action of well-conserved nucleases named Dicers or Dicer-like (DCL) proteins (Bologna and Voinnet 2014). The resulting sRNAs then associate with a family of sRNA-binding proteins called Argonautes (AGO) to form a ribonucleoprotein complex. This complex mediates regulation of diverse RNA substrates, at both transcriptional as well as posttranscriptional levels (Chen 2009; Mallory and Vaucheret 2010; Ma and Zhang 2018).
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