Role of PAP Smear Cytology and p 16 Immunocytochemistry for Detection of Cervical Lesions of Cervix: A Hospital-Based St
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ORIGINAL ARTICLE
Role of PAP Smear Cytology and p16 Immunocytochemistry for Detection of Cervical Lesions of Cervix: A Hospital-Based Study Gangotree Mohanty1
•
Ashok kumar Padhy2
Received: 8 September 2020 / Accepted: 18 November 2020 / Published online: 27 November 2020 Ó Association of Gynecologic Oncologists of India 2020
Abstract Back ground Pap-smear-based cytology is used widely in detecting cervical precancerous lesions and still needs further standardization. Co-expression of p16 can be used as an additional biomarker for detection of cervical lesions. Objectives The aim of the study was to assess the diagnostic value of Pap smears and detection of the p16 proteins in women with cervical lesions. Materials and Methods Totally 66 cases of cervical smear were taken, out of whom 14 samples were cytologically normal, 16 samples were inflammatory, 15 samples had low squamous intraepithelial lesions (LSIL), 11 samples had high squamous intraepithelial lesions (HSIL) and 10 samples had cervical squamous cell carcinoma (SCC). The expression of p16 was evaluated by immunohistochemical method. The immunostaining results of p16 were classified into four categories: negative, 1?, 2? and 3?. Results There was a significant increase in the expression of p16 (P \ 0.001) from cytologically normal to SCC. The expression of p16 (P = 0.01) was significantly increased in inflammatory, LSIL, HSIL and squamous cell carcinoma, and the results were compared with those of Pap smear cytology. Conclusion The result of p16 was compared with that of Pap smear cytology and can improve the diagnostic accuracy of cervical lesions. p16 expressions increase from inflammatory to LSIL, HSIL and SCC. Keywords LSIL HSIL Immunohistochemistry p16 Pap smear cytology
Introduction Cervical cancer is the second most common malignancy in women worldwide. Every year about 1,00,000 of new cases were detected, resulting in 80% of death [1]. The Pap smear cytology diagnosis of cervical biopsies that is often considered as the ‘‘gold standard’’ can be significantly hampered by intra- and inter-observer variability [2, 3]. Therefore, accurate diagnosis of cervical lesions is much more important. p16 is a cyclin-dependent kinase inhibitor
that regulates transition from the G1 phase to the S phase of the cell cycle [4, 5]. The value of p16 in diagnosis of cervical cancer has already been shown, but in regard to the relation of p16 with Pap smear cytology, no study was found. The aim of this study was to evaluate the role of p16 as prognostic markers and compare it with that of Pap smear cytology of cervical cancer.
Materials and Methods Collection of Samples
& Gangotree Mohanty [email protected] Ashok kumar Padhy [email protected] 1
Ravenshaw University, Cuttack, Odissa Pin-753003, India
2
Department of Gynaceology and Obstratics, Acarya Harihar Regional Cancer Centre, Cuttack, Odissa Pin-753003, India
A total of 66 patient samples were taken for this study who came to the outpatients department (OPD) of the hospital. Afte
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