Roles and mechanisms of leptin in osteogenic stimulation in cervical ossification of the posterior longitudinal ligament
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RESEARCH ARTICLE
Open Access
Roles and mechanisms of leptin in osteogenic stimulation in cervical ossification of the posterior longitudinal ligament Bin Feng†, Shiliang Cao†, Jiliang Zhai, Yi Ren, Jianhua Hu, Ye Tian* and Xisheng Weng*
Abstract Background: Hyperleptinemia is a common feature of obese people, and leptin, an adipocyte-derived cytokine, is believed to be an important factor in the pathogenesis of cervical ossification of the posterior longitudinal ligament(C-OPLL). So this research was to identify the relation between the serum leptin and bone metabolic markers and how the leptin induced osteogenic effect in C-OPLL. Methods: Sixty-four samples were selected to determine the concentration of leptin, insulin, and alkaline phosphatase. And the association of leptin with these factors was also examined. We also evaluate the effect of leptin on the development of C-OPLL and further explored the possible underlying mechanism in vitro. Results: We found that serum leptin concentrations were higher in females than in males. Serum leptin and ALP concentrations were increased significantly in C-OPLL females compared to non-OPLL females. In OPLL subjects, the serum leptin concentration corrected for body mass index correlated negatively with the ALP concentrations. In C-OPLL cells, leptin treatment led to a significant increase in mRNA expressions of ALP and OCN and formation of mineralized nodule. Our experiments reported here that osteogenic effect of leptin in C-OPLL cells could be mediated via ERK1/2, p38 MAPK, and/or JNK signaling pathways. Conclusions: From this research, we got that leptin treatment led to a significant increase in mRNA expressions of ALP and OCN and formation of mineralized nodule. And the osteogenic effect of leptin in C-OPLL cells could be mediated via ERK1/2, p38 MAPK, and/or JNK signaling pathways. Keywords: Leptin, Ossification of the posterior longitudinal ligament (OPLL), Gender, Bone metabolic markers, Signaling pathway
Background Ossification of the posterior longitudinal ligament (OPLL) is a common musculoskeletal disease, characterized by ectopic bone formation of the spinal ligament preferentially at the cervical spine [1, 2]. As OPLL commonly involved in cervical spine resulting in myelopathy or radiculopathy, in this condition, we said it cervical OPLL(C-OPLL). Enlarged ossified ligament compresses * Correspondence: [email protected]; [email protected] † Bin Feng and Shiliang Cao contributed equally to this work. Department of Orthopedics Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China
the spinal cord and nerve roots, eventually leads to the neurological deficit [3, 4]. Its prevalence is higher among Asian populations, and in Chinese populations, the average prevalence is reported to be 3.08% [5–8]. Although the exact pathogenesis of C-OPLL remains unclear, leptin is supposed to be an important factor in the pathogenesis of C-OPLL. Leptin, a product of the ob gene which is expresse
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