Root Silicon Treatment Modulates the Shoot Transcriptome in Brassica napus L. and in Particular Upregulates Genes Relate
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ORIGINAL PAPER
Root Silicon Treatment Modulates the Shoot Transcriptome in Brassica napus L. and in Particular Upregulates Genes Related to Ribosomes and Photosynthesis Philippe Etienne 1 & Jacques Trouverie 1 & Cylia Haddad 1 & Mustapha Arkoun 2 & Jean-Claude Yvin 2 & José Caïus 3,4 & Véronique Brunaud 3,4 & Philippe Laîné 1 Received: 8 June 2020 / Accepted: 9 September 2020 # Springer Nature B.V. 2020
Abstract While the benefit of silicon (Si) is often reported in cultivated plants, and particularly in those experiencing stress conditions, the underlying mechanisms are poorly described and controversial [1]. For a long time the mechanical role of Si in the cell wall has been considered as the main explanation for its beneficial effects in alleviating plant stresses. Another assumption is that a low proportion (around 1%) of the Si taken up by plants might modulate some metabolic pathways. Investigating this concept, Haddad et al. [2] demonstrated that in roots of Brassica napus plants, the modulation of a large range of genes by Si could explain an increased resistance to nitrogen (N) deficiency. However, since the Si benefit is mainly associated with maintaining photosynthetic activity and a delay in leaf senescence in the mature leaves of plants cultivated under N- deficiency [3], the aim of the present study was to identify (using a RNA sequencing approach) the transcriptomic modifications in the shoot compartment of +Si plants. Our results showed that there were 296 genes differentially expressed genes (DEGs)) in shoots of Brassica napus treated with Si (root supply 1.7 mM for 7 days). Among these genes, 19 and 31 upregulated genes were related to ribosomes and photosynthetic pathways, respectively. From these results, the assumption that a Si supply can facilitate efficient metabolic reinforcement and alleviate the biotic and abiotic stresses experienced by plants is discussed. Keywords Brassica napus . Photosynthetic pathway . Ribosomes . RNAseq . Shoot . Silicon
Philippe Etienne, Jacques Trouverie and Philippe Laîné contributed equally to this work. Doctoral fellowship co-funded by European Union, Normandy County Council, in the framework of the ERDF-ESF operational program 20142020 and the CMI group Roullier. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12633-020-00710-z) contains supplementary material, which is available to authorized users. * Philippe Etienne [email protected] 1
2
UNICAEN, INRAE, UMR 950 EVA, SFR Normandie Végétal (FED4277), Normandie Université, 14000 Caen, France Centre Mondial de l’Innovation Roullier, Laboratoire de Nutrition Végétale, 35400 Saint Malo, France
3
Institute of Plant Sciences Paris-Saclay (IPS2), CNRS, INRAE, Université Paris-Sud, Université d’Evry, Université Paris-Saclay, Bâtiment 630, Plateau de Moulon, 91192 Gif sur Yvette, France
4
Institute of Plant Sciences Paris-Saclay (IPS2), CNRS, INRAE Université Paris-Diderot, Sorbonne Paris-Cité, Bâtiment 630, Plateau de Moulon, 91192 Gif sur Yvette, Fra
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