RsbV of Listeria monocytogenes contributes to regulation of environmental stress and virulence
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ORIGINAL PAPER
RsbV of Listeria monocytogenes contributes to regulation of environmental stress and virulence Zaichao Zhang • Qingling Meng • Jun Qiao • Lihong Yang • Xuepeng Cai • Guanglei Wang Chuangfu Chen • Lijuan Zhang
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Received: 12 October 2012 / Revised: 31 October 2012 / Accepted: 14 November 2012 / Published online: 29 November 2012 Ó Springer-Verlag Berlin Heidelberg 2012
Abstract SigmaB factor is an important regulatory factor for stress response in Gram-positive bacteria such as Listeria monocytogenes (L. monocytogenes), Staphylococcus aureus and Bacillus subtilis. However, the activity of SigmaB factor is regulated by RsbV factor. Currently, the functional studies of RsbV factor are mostly focused on non-pathogenic B. subtilis, but the roles of RsbV factor in pathogenic L. monocytogenes during the regulation of environmental stress and virulence are still unclear. In the study, a DRsbV mutant of L. monocytogenes was constructed to explore the regulatory role of RsbV in environmental stress and virulence. The environmental stress experiments indicated that the growth and survival capability of DRsbV mutant obviously decreased in stress of low temperature, osmotic pressure, alcohol and acid, compared with EGD strain. The macrophage infection experiment indicated that DRsbV mutant had weaker survival capability than EGD strain, and the expression of Communicated by Erko Stackebrandt. Z. Zhang Q. Meng (&) J. Qiao (&) L. Yang G. Wang C. Chen College of Animal Science and Technology, Shihezi University, Shihezi 832003, Xinjiang, China e-mail: [email protected] J. Qiao e-mail: [email protected] Q. Meng X. Cai State Key Lab of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, Gansu, China L. Zhang Institute for Infectious Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
PrfA, actA, PlcA and LLO was down-regulated in infected cells. Animal inoculation experiments indicated that RsbV deletion significantly reduced the pathogenicity of L. monocytogenes. Our data demonstrate that, in addition to regulating tolerance under environmental stress conditions, RsbV also contributes to regulation of L. monocytogenes virulence. Keywords Listeria monocytogenes RsbV Stress tolerance Virulence
Introduction Listeria monocytogenes (L. monocytogenes), as an intracellular Gram-positive bacterium, is one of an important zoonotic pathogens. Infants, the elders, young animals are inclined to get infection of L. monocytogenes, which leads to a variety of diseases (Farber and Peterkin 1991; Ooi and Lorber 2005). The bacterium is considered as one of the four major foodborne pathogens (Swaminathan and Gerner-Smidt 2007). L. monocytogenes is widely present in natural environments such as water and soil, and has strong environmental adaptability and can survive in a variety of conditions with acids, high-salt and low-temperature environments (Chorianopoulos et al. 2011; Gorski e
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