Salivary alpha-1-antitrypsin and macrophage migration inhibitory factor may be potential prognostic biomarkers for oncol
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ORIGINAL ARTICLE
Salivary alpha-1-antitrypsin and macrophage migration inhibitory factor may be potential prognostic biomarkers for oncologic treatment–induced severe oral mucositis Natália Rangel Palmier 1 & Adriana Franco Paes Leme 2 & Tatiane De Rossi 2 & Guilherme Pimentel Telles 3 & Karina Morais-Faria 4 & Luiz Paulo Kowalski 5 & Gustavo Nader Marta 6,7 & Thaís Bianca Brandão 4,8 & Praveen R Arany 9 & César Augusto Migliorati 10 & Alan Roger Santos-Silva 1 & Ana Carolina Prado-Ribeiro 4 Received: 27 June 2020 / Accepted: 28 September 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Aims Evaluate the abundance of the selected targets, alpha-1-antitrypsin (A1AT) and macrophage migration inhibitory factor (MIF), and correlate these findings with the risk of developing severe oral mucositis (OM). Materials and methods Head and neck squamous cell carcinoma (HNSCC) patients submitted to radiotherapy (RT) or chemoradiotherapy (CRT) were assessed. OM grade and pain were evaluated daily during treatment. Two protein targets, A1AT and MIF, were evaluated, using selected reaction monitoring-mass spectrometry (SRM-MS), in whole saliva, collected prior to oncologic treatment. The results obtained from the targeted proteomic analysis were correlated with OM clinical outcomes. Results A total of 27 patients were included, of whom 21 (77.8%) had locally advanced disease (clinical stage III or IV). Most patients (70.4%) received CRT. OM grades 2 (40.8%) and 3 (33.3%) were the most prevalent during RT with a mean highest reported OM-related pain of 3.22 through the visual analogue scale (VAS). The abundance of A1AT and MIF correlated significantly with severe (grades 3 or 4, p < 0.02) compared with moderate-low (grades 1 or 2, p < 0.04) OM grade. Conclusions There is a correlation between the abundance of salivary A1AT and MIF and oncologic treatment–induced OM. The correlation of MIF expression with severe OM appears to be compatible with its physiological pro-inflammatory role. These results open up great possibilities for the use of salivary MIF and A1AT levels as prognostic markers for effective therapeutic interventions, such as photobiomodulation therapy, patient-controlled analgesia, or personalized medicaments. Keywords Head and neck neoplasms . Radiotherapy . Oral mucositis . Salivary proteins . Alpha 1-antitrypsin . Macrophage migration inhibitory factors
* Ana Carolina Prado-Ribeiro [email protected] Natália Rangel Palmier [email protected] Adriana Franco Paes Leme [email protected] Tatiane De Rossi [email protected] Guilherme Pimentel Telles [email protected] Karina Morais-Faria [email protected] Luiz Paulo Kowalski [email protected]
Gustavo Nader Marta [email protected] Thaís Bianca Brandão [email protected] Praveen R Arany [email protected] César Augusto Migliorati [email protected] Alan Roger Santos-Silva [email protected] Extended author information available on the last page of the article
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