Sample Preparation Handbook for Transmission Electron Microscopy Met
This two-volume Handbook is a comprehensive and authoritative guide to sample preparation for the transmission electron microscope. This first volume covers general theoretical and practical aspects of the methodologies used for TEM analysis and observati
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Jeanne Ayache · Luc Beaunier Jacqueline Boumendil · Gabrielle Ehret Dani`ele Laub
Sample Preparation Handbook for Transmission Electron Microscopy Methodology
Foreword by Ron Anderson
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Jeanne Ayache Institut Gustave Roussy Unité mixte CNRS-UMR8126-IGR Laboratoire de Microscopie Moléculaire et Cellulaire 39 rue Camille Desmoulin 94805 Villejuif CX France [email protected]
Luc Beaunier Université Paris VI UPR 15 CNRS Boîte courrier 133 Labo. Interfaces et Syst`emes Electrochimiques 4 place Jussieu 75252 Paris CX 05 France [email protected]
Jacqueline Boumendil Université Lyon I Centre de Microscopie Electronique Appliquée à la Biologie et à la Géologie 43 bd. du 11 Novembre 1918 69622 Villeurbanne CX France [email protected]
Gabrielle Ehret Université Strasbourg CNRS-UMR 7504 Inst. Physique et Chimie des Matériaux 22 rue du Loess 67034 Strasbourg CX 2 France [email protected]
Dani`ele Laub Ecole Polytechnique Fédérale de Lausanne Faculté des Sciences de Base Centre Interdisciplinaire de Microscopie Electronique 039 Station 12 1015 Lausanne Bâtiment MXC Switzerland [email protected]
ISBN 978-0-387-98181-9 e-ISBN 978-0-387-98182-6 DOI 10.1007/978-0-387-98182-6 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2010923800 © Springer Science+Business Media, LLC 2010 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Cover illustration: Conception: Dan Perez TEM image of freezing defects in a frozen thin film, showing clusters of segregated crystals along the holes of the carbon membrane. (Baconnais S., CNRS-UMR8126, Villejuif, FR). Printed on acid-free paper Springer is part of Springer Science+Business Media (www.springer.com)
The gift that microscopy brings us, beyond the beauty of the images, is that it gives us access to the Art of Matter and brings us to the heart of the mechanisms, “from the structure of inert matter to the complexity of the living.” There, where the infinitely small and the infinitely large come together. . . It is a lesson of life and humility. Jeanne Ayache
Foreword
Successful transmission electron microscopy in all of its manifestations depends on the quality of the specimens examined. Biological specimen preparation protocols have usually been more rigorous and time consuming than those in the physical sciences. For this reason, there has been a wealth of scientific literatu
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