SARS-CoV-2 binds platelet ACE2 to enhance thrombosis in COVID-19
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(2020) 13:120
RESEARCH
Open Access
SARS-CoV-2 binds platelet ACE2 to enhance thrombosis in COVID-19 Si Zhang2*†, Yangyang Liu1†, Xiaofang Wang1†, Li Yang3†, Haishan Li4, Yuyan Wang5, Mengduan Liu1, Xiaoyan Zhao1, Youhua Xie5, Yan Yang6, Shenghui Zhang7, Zhichao Fan8, Jianzeng Dong1, Zhenghong Yuan5, Zhongren Ding1, Yi Zhang3* and Liang Hu1*
Abstract Background: Critically ill patients diagnosed with COVID-19 may develop a pro-thrombotic state that places them at a dramatically increased lethal risk. Although platelet activation is critical for thrombosis and is responsible for the thrombotic events and cardiovascular complications, the role of platelets in the pathogenesis of COVID-19 remains unclear. Methods: Using platelets from healthy volunteers, non-COVID-19 and COVID-19 patients, as well as wild-type and hACE2 transgenic mice, we evaluated the changes in platelet and coagulation parameters in COVID-19 patients. We investigated ACE2 expression and direct effect of SARS-CoV-2 virus on platelets by RT-PCR, flow cytometry, Western blot, immunofluorescence, and platelet functional studies in vitro, FeCl3-induced thrombus formation in vivo, and thrombus formation under flow conditions ex vivo. (Continued on next page)
* Correspondence: [email protected]; [email protected]; [email protected] † Si Zhang, Yangyang Liu, Xiaofang Wang, and Li Yang contributed equally to this work. 2 Department of Biochemistry and Molecular Biology, NHC Key Laboratory of Glycoconjugates Research, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China 3 Biotherapy Center, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China 1 Department of Cardiology, the First Affiliated Hospital of Zhengzhou University, Cardiovascular Institute of Zhengzhou University, Zhengzhou 450052, China Full list of author information is available at the end of the article © The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
Zhang et al. Journal of Hematology & Oncology
(2020) 13:120
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