Secondary Metabolite Profiling Via LC-HRMS Q-TOF of Foleyola Billotii , an Endemic Brassicaceae Plant of North-Western S
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Pharmaceutical Chemistry Journal, Vol. 54, No. 7, October, 2020 (Russian Original Vol. 54, No. 7, July, 2020)
SECONDARY METABOLITE PROFILING VIA LC-HRMS Q-TOF OF Foleyola billotii, AN ENDEMIC BRASSICACEAE PLANT OF NORTH-WESTERN SAHARA Yazid Mahdaoui,1,* François Perreau,2 Houria Hadj-Arab,3 and Aicha Belkebir1 Original article submitted March 8, 2020. LC-HRMS Q-TOF method was used to identify and quantify metabolites of Foleyola billotii Maire plant. A series of 15 compounds were tentatively identified: three glucosinolates, 2 aliphatic (progoitrin and gluconapin) and 1 indolic (glucobrassicin); six chlorogenic acids, four coumaroyl quinic acid (CoQA) isomers and two feruloyl quinic acid (FQA) isomers; two diglycosylated flavonols, one quercetin-derived and one kaempferol-derived; three sinapic acid derivatives, sinapoyl threonic acid, hexose sinapate and a third derivative supposedly attributed to a feruloyl quinic acid sinapate; and finally, a glycosylated trans-cinnamic acid which could correspond to feruloylthreonic acid (FTA). Glucosinolates content was 55 times higher in cultivated plants; chlorogenic acids and flavonols increased too by 5 and 3.5 times, respectively. In contrast, sinapates decreased by 3 times in cultivated plants, this last result can be explained by reduced UV-B intensity in the north of Algeria. The change in F. billotii compound profile was more quantitative rather than qualitative. Keywords: Foleyola billotii, Brassicaceae; LC-HRMS Q-TOF; glucosinolates; flavonols; chlorogenic acids; sinapates; antioxidants.
of these multiple environmental stresses is the formation of an important diversity concerning the defence mechanisms [2]. In particular, members of the Brassicaceae family are known to contain a variety of secondary metabolites including lignans, alkaloids, terpenes, glucosinolates (GLs) and polyphenolic compounds involved in plant defence mechanisms [3 – 5]. GLs are sulfur-containing secondary metabolites characteristic of the Brassicaceae family, having a specific structure with unusual glucose-sulfur bonds [6]. Their hydrolysis results in various bioactive derivatives, in particular, isothiocyanates with skeleton consisting of a sugar (D-glucopyranosyl), a thiohydroximate anomeric O-sulphated function, and a variable side chain that has amino acids as precursors. In recent years, GL molecules have been particularly studied for their important biological properties and important role in the plant defence system against biotic threats [7, 8]. Their level increases in case of osmotic (salt or water) stresses and is implicated in the process of osmotic adjustment [9]. In addition, GLs can have a preventive role in human health and may be beneficial against different pathologies such as cancer, cardiovascular and neurodegenerative diseases [10, 11].
1. INTRODUCTION Foleyola billotii Maire is a woody plant belonging to the Brassicaceae family, formerly known as Cruciferae. Member of the tribe of Brassiceae [1], it is endemic to the south of both Algeria and Morocco. This endangered
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