Single Molecule and Single Cell Sequencing
This book presents an overview of the recent technologies in single molecule and single cell sequencing. These sequencing technologies are revolutionizing the way of the genomic studies and the understanding of complex biological systems. The PacBio seque
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Yutaka Suzuki Editor
Single Molecule and Single Cell Sequencing
Advances in Experimental Medicine and Biology Volume 1129
Editorial Board IRUN R. COHEN, The Weizmann Institute of Science, Rehovot, Israel ABEL LAJTHA, N.S. Kline Institute for Psychiatric Research, Orangeburg, NY, USA JOHN D. LAMBRIS, University of Pennsylvania, Philadelphia, PA, USA RODOLFO PAOLETTI, University of Milan, Milan, Italy NIMA REZAEI, Children’s Medical Center Hospital, Tehran University of Medical Sciences, Tehran, Iran
More information about this series at http://www.springer.com/series/5584
Yutaka Suzuki Editor
Single Molecule and Single Cell Sequencing
Editor Yutaka Suzuki Department of Computational Biology and Medical Sciences University of Tokyo Chiba, Chiba, Japan
ISSN 0065-2598 ISSN 2214-8019 (electronic) Advances in Experimental Medicine and Biology ISBN 978-981-13-6036-7 ISBN 978-981-13-6037-4 (eBook) https://doi.org/10.1007/978-981-13-6037-4 Library of Congress Control Number: 2019934708 © Springer Nature Singapore Pte Ltd. 2019 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. This Springer imprint is published by the registered company Springer Nature Singapore Pte Ltd. The registered company address is: 152 Beach Road, #21-01/04 Gateway East, Singapore 189721, Singapore
Contents
Strategies for Converting RNA to Amplifiable cDNA for Single-Cell RNA Sequencing Methods���������������������������������������������������� 1 Yohei Sasagawa, Tetsutaro Hayashi, and Itoshi Nikaido Integrated Fluidic Circuits for Single-Cell Omics and Multi-omics Applications ������������������������������������������������������������������������ 19 Mark Lynch and Naveen Ramalingam Single-Cell DNA-Seq and RNA-Seq in Cancer Using the C1 System���������������������������������������������������������������������������������������� 27 Masahide Seki, Ayako Suzuki, Sa
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