Spectroscopic Analysis of Fluorescent Proteins Infiltrated into Photonic Crystals
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PTICAL MATERIALS
Spectroscopic Analysis of Fluorescent Proteins Infiltrated into Photonic Crystals N. Zhdanovaa, *, A. Pakhomovb, S. Rodionovc, Yu. Strokovaa, S. Svyakhovskiya, and A. Saletskiia a Lomonosov
Moscow State University, Moscow, 119991 Russia “Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997 Russia c N.N. Priorov Central Institute for Traumatology and Orthopedics, Moscow, 127299 Russia *e-mail: [email protected]
b Shemyakin
Received December 16, 2019; revised January 19, 2020; accepted February 27, 2020
Abstract—Spectral properties of enhanced-green fluorescent protein and monomeric red fluorescent protein in porous photonic structures have been studied. The fluorescent proteins were successfully infiltrated into porous silicon photonic structures with different positions of the photonic band gap in visible spectral range. The intensity of fluorescence is enhanced in the spectral regions of high photonic density of states. The possibility to control the fluorescence spectra by the structure with the photonic band gap is demonstrated. Keywords: photonic crystals, porous silicon, fluorescent proteins, photonic band gap DOI: 10.1134/S0030400X20070267
1. INTRODUCTION The infiltration of fluorescent macromolecules into porous photonic materials is one of the hot topics that connects bioscience and nanophotonics [1]. Fluorescent proteins (FPs) belong to a well-known type of macromolecules with chromophore buried inside a beta-barrel with diameter and height about 2.4 and 4.2 nm, respectively. Depending on the chromophore structure the position of maximum of emission spectrum varies from 445 to 655 nm [2]. The fluorescent properties of composite structures made of porous media filled with functional materials have been studied in various cases such as fluorescent quantum dots in colloidal photonic crystals [3] and proteins in photonic crystal fibers [4]. Biological materials can be used as active laser media in cavities [5]. Studies have shown that fluorescent properties of proteins can be driven by the photonic band gap (PBG) structures and the colour of fluorescence can be governed by the spectral position of the photonic band gap [6]. In mentioned works it was shown that the fluorescence spectrum can be artificially modified by the photonic band gap due to Purcell effect and modification of the photonic density of states within the photonic crystal. We expect that these phenomena can be enhanced by using high-quality photonic crystals. We suggest porous silicon photonic crystals as a materials with arbitrary position of the photonic band gap. In such materials the fluorescence spectrum can be governed more efficiently.
In this work we chose a pair of visible fluorescent proteins (visFPs): enhanced green fluorescent protein (EGFP) and monomeric red fluorescent protein (TagRFP)—and infiltrated them into photonic crystals of different spectral positions of the PBG. The fluorescence spectra of proteins within the porous photonic structure were measured. 2. MAT
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