Studies on Trueperella pyogenes isolated from an okapi ( Okapia johnstoni ) and a royal python ( Python regius )
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CASE REPORT
Open Access
Studies on Trueperella pyogenes isolated from an okapi (Okapia johnstoni) and a royal python (Python regius) Marwa F. E. Ahmed1, Mazen Alssahen2, Christoph Lämmler2, Tobias Eisenberg3, Madeleine Plötz4 and Amir Abdulmawjood4*
Abstract Background: The present study was designed to characterize phenotypically and genotypically two Trueperella pyogenes strains isolated from an okapi (Okapia johnstoni) and a royal python (Python regius). Case presentation: The species identity could be confirmed by phenotypic properties, by MALDI-TOF MS analysis and by detection of T. pyogenes chaperonin-encoding gene cpn60 with a previously developed loop-mediated isothermal amplification (LAMP) assay. Furthermore, sequencing of the 16S ribosomal RNA (rRNA) gene, the 16S-23S rDNA intergenic spacer region (ISR), the target genes rpoB encoding the β-subunit of bacterial RNA polymerase, tuf encoding elongation factor tu and plo encoding the putative virulence factor pyolysin allowed the identification of both T. pyogenes isolates at species level. Conclusions: Both strains could be clearly identified as T. pyogenes. The T. pyogenes strain isolated in high number from the vaginal discharge of an okapi seems to be of importance for the infectious process; the T. pyogenes strain from the royal python could be isolated from an apparently non-infectious process. However, both strains represent the first isolation of T. pyogenes from these animal species. Keywords: 16S rRNA gene, 16S-23S rDNA intergenic spacer region, LAMP, MALDI-TOF MS, pyolysin, rpoB, tuf
Background Trueperella pyogenes is worldwide considered as part of the commensal biota of skin and mucous membranes of the upper respiratory and urogenital tract of animals [1]. However, T. pyogenes is also an important opportunistic pathogen that causes mastitis, abortion and a variety of diverse pyogenic infections in livestock, including cattle, sheep, goats, horses, and pigs [2–4]. In cattle, T. pyogenes appears to be responsible for infections of the reproductive tract [5] and the mammary gland [6], as well as cases of pneumonia and liver abscessation of large * Correspondence: [email protected] 4 Institute of Food Quality and Food Safety, Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Bischofsholer Damm 15, D-30173 Hannover, Germany Full list of author information is available at the end of the article
and small ruminants [7]. In swine, T. pyogenes is well known as a causative agent of different types of inflammation in various organs including the lung, heart, joints, mammary glands, and in the reproductive tract [8, 9]. Furthermore, T. pyogenes could be found in companion animals [4]. One of the first reported cases in companion animals was an otitis externa detected in a cat and cystitis in a dog [10]. More recently, Wareth et al. [11] described a co-infection case of T. pyogenes with Brucella abortus in a cat and dog. Additionally, various wildlife animals could harbour T. pyogen
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