Study of Blood Serum in Rats with Transplanted Cholangiocarcinoma Using Raman Spectroscopy
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Study of Blood Serum in Rats with Transplanted Cholangiocarcinoma Using Raman Spectroscopy A. A. Mankovaa, O. P. Cherkasovab, c, *, E. N. Lazarevad, e, A. B. Bucharskayaf, P. A. Dyachenkod, e, Yu. V. Kistenevd, g, D. A. Vrazhnovd, h, V. E. Skibad, V. V. Tuchind, e, i, and A. P. Shkurinova, c a
Moscow State University, Moscow, 119991 Russia Institute of Laser Physics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090 Russia c Institute for Laser and Information Technology Problems of RAS—Branch of the Federal Research Center for Crystallography and Photonics of RAS, Shatura, 140700 Russia d National Research Tomsk State University, Tomsk, 634050 Russia e Saratov National Research State University, Saratov, 410012 Russia f Saratov State Medical University, Saratov, 410012 Russia g Siberian State Medical University, Tomsk, 634050 Russia h Institute of Strength Physics and Materials Science, Siberian Branch, Russian Academy of Sciences, Tomsk, 634055 Russia i Institute of Precision Mechanics and Control, Russian Academy of Sciences, Saratov, 410028 Russia *E-mail: [email protected] b
Received January 10, 2020; revised January 31, 2020; accepted February 28, 2020
Serum samples of healthy rats and rats with inoculated tumors on days 14 and 28 after transplantation of cholangiocarcinoma cells were analyzed using Raman spectroscopy (RS). It was shown that the intensity of the amide 1 band differs in the serum spectra depending on the stage of tumor development and correlates with the protein concentration in the samples. In some animals, there is a significant increase in the blood glucose concentration, leading to glycation of proteins and a change in the shape of the amide 1 band. The appearance of a band at 1670 cm–1 is most pronounced in the spectra of samples on the 28th day of the experiment and may be associated with an increase in the content of β-structural elements in the protein conformation. Using the principal component analysis, we assessed the general differences in the Raman spectra of the blood serum in rats of control groups and rats on the 28th day after tumor transplantation. Keywords: Raman spectroscopy, blood serum, experimental liver cancer, principal component analysis DOI: 10.1134/S0030400X20070115
INTRODUCTION The study of integral indicators characterizing the differences between biological samples obtained from healthy and sick people is of great importance, since diagnostics based on a single marker are often uninformative [1, 2]. Raman spectroscopy (RS) is a powerful analytical method that can be used in the differential diagnosis of diseases [3]. The Raman spectrum is a molecular imprint of the sample and gives quantitative information about its chemical composition [4]. Biochemical changes in cells and tissues associated with various diseases can lead to significant changes in the Raman spectra [3–5]. The use of RS for the differential diagnosis of cancer of the brain, breast, skin and several other types of oncology is described in [6]. A blood test can pr
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