Subtractive Hybridization and cDNA Cloning
There has long been a desire among scientists, investigating the subtle changes that occur in cells as they grow and develop, to identify with precision, the transcripts that are newly transcribed as a result of altered physiology. Whether in response to
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Springer-Verlag Berlin Heidelberg GmbH
R. CURTIS BIRD
BRUCE F. SMITH (EDS.)
Genetic Library Construction and Screening Advanced Techniques and Applications With 30 Figures
Springer
DR.
R. CURTIS BIRO
DR. BRUCE
F. SMITH
Department of Pathobiology Auburn University Auburn,AL 36849-5519 USA
Scott-Ritchey Research Center Auburn University Auburn, AL 36849 USA
e-mail: [email protected]
e-mail: [email protected]
Applied for Library of Congress Cataloging-in-Publication Data Die Oeutsche Bibliothek - CIP-Einheitsaufnahme Genetic library construction and screening : advanced techniques and applications / R. Curtis Bird; Bruce F. Smith (ed.). - Berlin; Heidelberg ; New York ; Barcelona; Hong Kong; London; Milan; Paris; Tokyo: Springer, 2002 (Springer lab manuals) ISBN 978-3-642-47733-1 ISBN 978-3-642-56408-6 (eBook) DOI 10.1007/978-3-642-56408-6 This work is subject to copyright. All rights are reserved, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilm or in any other way, and storage in data banks. Ouplication of this publication or parts thereof is permitted only under the provisions of the German Copyright Law of September 9, 1965, in its current version, and permissions for use must always be obtained from Springer-Verlag. Violations are liable for prosecution under the German Copyright Law.
http://www.springer.de © Springer-Verlag Berlin Heidelberg 2002 Originally published by Springer-Verlag Berlin Heidelberg New York in 2002 The use of general descriptive names, registered names, trademarks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. Product liability: The publisher cannot guarantee the accuracy of any information about dosage and application thereof contained in this book. In every individual case the user must check such information by consulting the relevant literature. Cover design: design & production GmbH, 0-69121 Heidelberg Typesetting: Kriiner, D-69115 Heidelberg SPIN 10531590
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Preface
In recent years, it has seemed as though classical cloning strategies were taking something of a back seat to new advances in the development of applied polymerase chain reaction strategies. The tried and established methods of library construction and recombinant DNAcloning pioneered during the 1970sand early 1980s provided many of the bedrock approaches used to find cDNA and genomic clones of significance and made possible accessible DNA sequencing technologies that did not require exotic or even hazardous chemistry. Today, these technologies are enjoying a renaissance as they find new utility in novel guises combined with the newer approaches and technologies based on polymerase chain reaction. Although it is unlikely that any scientist today would employ all of t
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