Synovial lymphoid neogenesis is associated with IL-23 expression and disease activity in rheumatoid arthritis
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POSTER PRESENTATION
Open Access
Synovial lymphoid neogenesis is associated with IL-23 expression and disease activity in rheumatoid arthritis Juan D Cañete1*, Raquel Celis1, Julio Ramírez1, Sara Marsal2, Gabriela Ávila2, Raimón Sanmartí1, José L Pablos3 From 6th European Workshop on Immune-Mediated Inflammatory Diseases Nice, France. 23-25 November 2011 Objective We analyzed whether LN is associated with specific patterns of inflammatory cytokine expression in synovial tissue (ST), and the potential association of cytokine expression with disease activity and response to therapy. Methods ST samples were obtained by arthroscopy from the inflamed knee of 62 RA patients. A second biopsy was obtained after a mean of 8±5 months of treatment in 21 patients who started TNF blockers. ST samples were immunostained with CD3 (T cell), CD20 (B cell), and MECA-79 (high endothelial vessels). Total ST mRNA was extracted and gene expression of CCR7, LT-beta, IL-7, IL10, IL-17A, IL-21, IL-22, IL-23, TNF-alpha, IL-1b, and IL6 was measured by quantitative real-time PCR. Clinical and biological data were collected at inclusion and after a median follow-up of 2.3 years. Results 28 out of 62 patients (45.2%) had LN, which was associated with a significantly-higher expression of three key molecular markers of LN: CCR7, IL-7 and LT-beta (p
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