Telomerase Activity Increased and Telomere Length Shortened in Peripheral Blood Cells from Patients with Immune Thromboc
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ORIGINAL RESEARCH
Telomerase Activity Increased and Telomere Length Shortened in Peripheral Blood Cells from Patients with Immune Thrombocytopenia Aiping Qi & Hu Zhou & Zeping Zhou & Xin Huang & Li Ma & Hongmei Wang & Yanhui Yang & Donglei Zhang & Huyuan Li & Ruimin Ren & Renchi Yang Received: 17 November 2011 / Accepted: 3 December 2012 / Published online: 14 December 2012 # Springer Science+Business Media New York 2012
Abstract Objectives To evaluate the telomere/telomerase system and its clinical significance in immune thrombocytopenia (ITP) patients. Methods A total of 237 ITP patients, 20 SLE patients and 200 age-and sex-matched healthy controls were included in this study. CD4+, CD8+ and CD19+ lymphocytes were purified by magnetic beads sorting from peripheral blood of 37 active chronic ITP patients and 22 age-and sex-matched healthy controls. Telomerase activity was assayed by Telo TTAGGG Telomerase PCR ELISA KIT. The relative telomere length of peripheral blood mononuclear cell (PBMC) was measured by a quantitative polymerase chain reactionbased method (Q-PCR) from 200 ITP patients and 178 ageand sex-matched healthy controls. A. Qi : H. Zhou : L. Ma : H. Wang : Y. Yang : D. Zhang : H. Li : R. Yang (*) State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 288 Nanjing Road, Tianjin 300020, People’s Republic of China e-mail: [email protected] Z. Zhou Department of Hematology, The Second Affiliated Hospital of Kunming Medical University, Kunming 650101, People’s Republic of China X. Huang Institute of Laboratory Animal Sciences and Max-Planck-Partner Group on Stem Cell Aging, Chinese Academy of Medical Sciences, Beijing 100021, China R. Ren Department of Surgery, Hospital of Tianjin Port, Tianjin 300456, China
Results Telomerase activity was increased in CD4+, CD8+ and CD19+ lymphocytes from ITP patients compared to those from healthy controls (p00.000). The level of telomerase activity in CD19+ lymphocyte was higher than those in CD4+ and CD8+ lymphocytes. Telomerase activity of CD19+ lymphocytes had a modest negative correlation with platelet count in ITP patients (p00.042). The relative telomere length of PBMC in ITP patients was significantly shorter than that in the healthy controls (p 00.002). Telomere length of PBMC in active ITP patients was significantly shorter than that in the controls (p00.000) and a tendency to be shorter even in inactive ITP patients (p0 0.065). Moreover, the telomere length in refractory and nonrefractory ITP patients were both significantly shorter than that in the controls (p00.025; p00.000). However no significant difference in telomere length of PBMC was found between refractory ITP patients and non-refractory ITP patients (p00.234). Conclusion An abnormal regulating telomere/telomerase system might be involved in the pathogenesis of ITP. Further studies may elucidate whether the telomere length could be considered as a predictive biomarker for the prognosis
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