The Collection of Zoosporic Eufungi at the University of Michigan (CZEUM): introducing a new repository of barcoded Chyt
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RESEARCH
IMA Fungus Open Access
The Collection of Zoosporic Eufungi at the University of Michigan (CZEUM): introducing a new repository of barcoded Chytridiomyceta and Blastocladiomycota cultures D. Rabern Simmons1, Anne E. Bonds1, Buck T. Castillo1, Rebecca A. Clemons1, Alex D. Glasco1, Jillian M. Myers1, Natasha Thapa1, Peter M. Letcher2, Martha J. Powell2, Joyce E. Longcore3 and Timothy Y. James1*
Abstract We formed the Collection of Zoosporic Eufungi at the University of Michigan (CZEUM) in 2018 as a cryopreserved fungal collection consolidating the University of Maine Culture Collection (UMCC, or JEL), the University of Alabama Chytrid Culture Collection (UACCC), and additional zoosporic eufungal accessions. The CZEUM is established as a community resource containing 1045 cryopreserved cultures of Chytridiomycota, Monoblepharidomycota, and Blastocladiomycota, with 52 cultures being ex-type strains. We molecularly characterized 431 cultures by amplifying the majority of the rDNA operon in a single reaction, yielding an average fragment length of 4739 bp. We sequenced multiplexed samples with an Oxford Nanopore Technology MinION device and software, and demonstrate the method is accurate by producing sequences identical to published Sanger sequences. With these data, we generated a phylogeny of 882 zoosporic eufungi strains to produce the most comprehensive phylogeny of these taxa to date. The CZEUM is thus largely characterized by molecular data, which can guide instructors and researchers on future studies of these organisms. Cultures from the CZEUM can be purchased through an online portal. Keywords: Cryopreservation, Oxford Nanopore, Phylogeny
Introduction Zoosporic eufungi are members of Kingdom Fungi that reproduce with flagellated spores. The zoosporic fungi form a diverse paraphyletic assemblage at the base of the fungal phylogeny and comprise the superphylum Chytridiomyceta (phyla Chytridiomycota, Monoblepharidomycota, and Neocallimastigomycota), and the phyla Blastocladiomycota, Olpidiomycota, and Rozellomycota/ Cryptomycota (Tedersoo et al. 2018; Naranjo-Ortiz and * Correspondence: [email protected] 1 Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, MI 48109, USA Full list of author information is available at the end of the article
Gabaldón 2019). Collectively, these microscopic fungi that reproduce with zoospores produced in a sporangium are often referred to as chytrids (Greek for “little pot”). In 2018 the Collection of Zoosporic Eufungi at the University of Michigan (CZEUM) was established with the aim of safeguarding historical culture collections from the two most active research labs in chytrid systematics over the last 30 years. Because these cultures resided in personal collections, the goal was to create a repository with infrastructure that would persist and facilitate continued research on the group. Support from the U. S. National Science Foundation’s program Collections in Support of Biological Research made the
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