The effect of suramin on inhibiting fibroblast proliferation and preventing epidural fibrosis after laminectomy in rats
- PDF / 1,688,461 Bytes
- 8 Pages / 595.276 x 790.866 pts Page_size
- 54 Downloads / 188 Views
RESEARCH ARTICLE
Open Access
The effect of suramin on inhibiting fibroblast proliferation and preventing epidural fibrosis after laminectomy in rats Jihang Dai†, Xiaolei Li†, Lianqi Yan, Hui Chen, Jun He, Shuguang Wang, Jingcheng Wang* and Yu Sun*
Abstract Background: Epidural fibrosis often causes serious complications in patients after lumbar laminectomy and discectomy and is associated with the proliferation of fibroblasts. Suramin is known to have an obvious inhibitory effect on the coactions of many growth factors and their receptors, but little was previously known about the effect of suramin on fibroblast proliferation and the progress of epidural fibrosis. Methods: We illustrated the effect of suramin on cultured fibroblasts of rats with different concentrations (0, 200, 400, 600 mg/l). The proliferation of suramin-treated fibroblasts was evaluated by CCK-8 and western blot analysis. Additionally, in a rat model of laminectomy, different concentrations of suramin (100, 200, and 300 mg/ml) and saline were applied to the laminectomy sites locally. The effect of suramin on preventing epidural fibrosis was detected by the Rydell classification, hydroxyproline content, histological analysis, and collagen density analyses. Results: The results of CCK-8 shown that suramin could significantly inhibit fibroblasts proliferation in a dose-dependent manner. The western blotting shown that the expression levels of the cell proliferation markers such as cyclin D1, cyclin E, and PCNA were down-regulated. Moreover, in a rat model, we found that suramin could reduce epidural fibrosis as well as inhibit fibroblast proliferation, and 300 mg/ml suramin had better effect. Conclusions: Topical application of suramin could reduce epidural fibrosis after laminectomy, and the application of suramin could inhibit the proliferation of fibroblasts in rats. This study indicates that suramin is a potent antifibrotic agent that may have therapeutic potential for patients with epidural fibrosis. Keywords: Suramin, Fibroblasts proliferation, Epidural fibrosis
Background Epidural fibrosis following lumbar laminectomy and discectomy is a common outcome and remains a challenging clinical problem for surgeons [1, 2]. It may result in unfavorable clinical outcome and contribute to more complications in revision spine surgery, such as dural tears, nerve root injury, and bleeding. Although the definite mechanism of fibrosis formation is still unclear, some reports have shown that fibroblast proliferation and the succeeding release of extracellular matrix were the main reasons for epidural fibrosis [3].
* Correspondence: [email protected]; [email protected] † Equal contributors Department of Orthopedics, Clinical Medical College of Yangzhou University, Orthopaedic Institute, Subei People’s Hospital of Jiangsu Province, Yangzhou 225001, China
At present, the process of epidural fibrosis cannot be effectively prevented. In order to control epidural fibrosis, many therapeutic strategies have been sought to reduce tissue damage and avoid adverse
Data Loading...