The evaluation of the transport medium for extracted premolars prior to cryopreservation: a systematic literature review
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The evaluation of the transport medium for extracted premolars prior to cryopreservation: a systematic literature review Noe¨mi M. C. De Roo Guy A. M. De Pauw
. Laurent A. M. Thierens
. Liesbeth Temmerman .
Received: 28 November 2017 / Accepted: 27 February 2018 Springer Science+Business Media B.V., part of Springer Nature 2018
Abstract Prior to cryopreservation, a tooth is transported from a contaminated oral environment to the tooth bank. Our objective was to identify all studies reporting or investigating a transport protocol prior to the cryopreservation of teeth, in terms of decontamination of the subjects. The systematic literature search (1970–2017) was based on MEDLINE via PubMed, Web of Science and the Cochrane Library. The reference lists of the included studies and the Science Citation Index were used for hand searching (snowballing). Only studies reporting the transport conditions of the transplant were included. Language restrictions for English, Dutch or French were applied. The search led to 14 eligible studies. Almost all studies
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10561-018-9690-5) contains supplementary material, which is available to authorized users. N. M. C. De Roo (&) L. A. M. Thierens L. Temmerman G. A. M. De Pauw Department of Orthodontics, School of Dental Medicine, Ghent University, C. Heymanslaan 10, P8, 9000 Ghent, Belgium e-mail: [email protected] L. A. M. Thierens e-mail: [email protected] L. Temmerman e-mail: [email protected] G. A. M. De Pauw e-mail: [email protected]
were laboratory studies, so the methodological quality of evidence was low. The majority of the included studies was performed by only five different research groups and the number of subjects varied between 1 and 120 teeth. In general, the teeth were stored in a tissue culture medium supplemented with fetal calf serum and/or different combinations of antibiotics and/or antimycotics. The teeth were transported cooled (4 C) or at room temperature, for a period of time not exceeding 24 h. Only three studies reported the irrigation of the teeth with phosphate buffered saline prior to the transport. The optimisation of the decontamination during transport was investigated in three studies (from 1971, 1980 and 1982). It was concluded that the literature on this topic is scarce, and the decontamination protocol for teeth, prior to cryopreservation has not been validated recently. Keywords Autotransplantation Cryopreservation Transport Tissue culture media Decontamination Tooth bank
Introduction Autotransplantation of premolars has become an accepted method to replace missing teeth. It is defined as the transplantation of teeth from one site to another in the same individual into extraction sockets or surgically prepared sites (Tsurumachi and Kuno
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Cell Tissue Bank
2011). The procedure can be indicated in patients with tooth loss due to trauma, caries, ectopic position, agenesis or developmenta
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