The first determination of Trichuris sp. from roe deer by amplification and sequenation of the ITS1-5.8S-ITS2 segment of
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ORIGINAL PAPER
The first determination of Trichuris sp. from roe deer by amplification and sequenation of the ITS1-5.8S-ITS2 segment of ribosomal DNA O. Salaba & K. Rylková & J. Vadlejch & M. Petrtýl & Š. Scháňková & A. Brožová & I. Jankovská & L. Jebavý & I. Langrová
Received: 23 August 2012 / Accepted: 21 November 2012 / Published online: 14 December 2012 # Springer-Verlag Berlin Heidelberg 2012
Abstract Trichuris nematodes were isolated from roe deer (Capreolus capreolus). At first, nematodes were determined using morphological and biometrical methods. Subsequently genomic DNA was isolated and the ITS15.8S-ITS2 segment from ribosomal DNA (RNA) was amplified and sequenced using PCR techniques. With u sing morphological and biometrical methods, female nematodes were identified as Trichuris globulosa, and the only male was identified as Trichuris ovis. The females were classified into four morphotypes. However, analysis of the internal transcribed spacers (ITS1-5.8S-ITS2) of specimens did not confirm this classification. Moreover, the female individuals morphologically determined as T. globulosa were molecularly identified as Trichuris discolor. In the case of the only male molecular analysis match the result of the molecular identification. Furthermore, a comparative phylogenetic study was carried out with the ITS1 and ITS2 sequences of the Trichuris species from various hosts. A comparison of biometric information from T. discolor individuals from this study was also conducted. O. Salaba : K. Rylková : J. Vadlejch : M. Petrtýl : Š. Scháňková : A. Brožová : I. Jankovská : I. Langrová (*) Department of Zoology and Fisheries, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences in Prague, Kamycka 129, 165 21 Prague 6-Suchdol, Czech Republic e-mail: [email protected] L. Jebavý Department of Husbandry and Ethology of Animals, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences in Prague, Kamycka 129, 165 21 Prague 6-Suchdol, Czech Republic
Introduction Whipworms of the genus Trichuris are very common nematodes of small ruminants, and the prevalence of whipworms in Europe, e.g. in sheep, varies from the 41.8 % to 100 % (Rehbein et al. 1996; Umur and Yukari 2005; Pedreira et al. 2006). According to previous studies of various authors, four species of Trichuris are known to parasitize in the caecum of sheep and goats in Europe: Trichuris ovis (Abilgaard 1795), Trichuris globulosa (Linstow 1901), Trichuris skrjabini (Baskakov 1924) and Trichuris capreoli (Artjuch 1948; Barth and Dollinger 1975; Baruš et al. 1977, 1978; Špakulová 1994; Cutillas et al. 1995, 1996, 2004; Oliveros et al. 2000; Callejón et al. 2008; Callejón et al. 2012). T. ovis, T. globulosa and Trichuris discolor (Linstow 1906) have been reported in cattle worldwide (Ortlepp 1937; Sarwar 1945, 1957; Knight 1971; Tenora et al. 1992; Lanfredi et al. 1995; Callejón et al. 2012). Whipworms are also often found in roe deer with a prevalence of 5–100 % (Dróždž et al. 1992; Rehbein et al. 2000,
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