The role of BAMBI in regulating adipogenesis and myogenesis and the association between its polymorphisms and growth tra
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ORIGINAL ARTICLE
The role of BAMBI in regulating adipogenesis and myogenesis and the association between its polymorphisms and growth traits in cattle Xinran Yang1 · Yue Ning2 · Chugang Mei1,3 · Weiyi Zhang1 · Jingchun Sun1 · Sihu Wang1 · Linsen Zan1,3 Received: 5 January 2020 / Accepted: 17 July 2020 © Springer Nature B.V. 2020
Abstract Bone morphogenic protein and activin membrane-bound inhibitor (BAMBI) is a transmembrane protein that affects the growth, development and muscle regeneration of the body by regulating the TGF-β, BMP and Wnt signaling pathways. Studies have found that BAMBI has important regulatory functions in skeletal muscle and preadipocytes in vivo and in vitro. However, research on this protein in cattle is lacking. In this study, to determine the role of BAMBI in the growth and development of cattle, we first found that the expression of BAMBI in adipose tissue and longissimus muscle of newborn and adult Qinchuan beef cattle was significantly different. Then we showed that BAMBI knockdown promoted the differentiation of bovine preadipocytes and suppressed myoblast myogenesis, as indicated by the increased lipid droplets and the decreased myotubes, as well as the corresponding significant changes in the expression of PPARγ, C/EBPα, C/EBPβ, FABP4, MyoD, MyoG and Myf6. Finally, to further verify the effect of BAMBI on the growth performance of cattle, we identified seven novel SNPs in the BAMBI genomic region, which were significantly correlated with one or more growth traits (p
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