The role of long non-coding RNA MALAT1 in patients with bipolar disorder
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ORIGINAL ARTICLE
The role of long non-coding RNA MALAT1 in patients with bipolar disorder Zeinab Shirvani Farsani 1 Bahar Naghavi Gargari 5
&
Alireza Zahirodin 2 & Sayyed Mohammad Hossein Ghaderian 3 & Jamal Shams 4 &
Received: 19 February 2020 / Accepted: 11 May 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Bipolar disorders are known as chronic, recurrent, and heterogenic diseases. Regarding, diagnosis and treatment of them are very complex. The molecular mechanism and pathophysiology of bipolar disorder are slightly known. Accordingly, long noncoding RNAs are considered as one of the main factors that are dysfunctional in many diseases such as the nervous system diseases. Hence, we aim to investigate the expression of two long non coding RNAs, MALAT1 and UCA1, in patients in bipolar disorder. The levels of MALAT1 and UCA1 lncRNA were evaluated in peripheral blood mononuclear cells (PBMCs) of 50 bipolar patients and 50 healthy controls with real-time PCR. Also, ROC curve analysis and correlation analysis were performed between the gene expression and some clinical features of bipolar individuals. The significant decline of MALAT1 expression level was found in the patients compared to controls; but no significant difference was observed in the UCA1 expression level between the patients and controls. Furthermore, computational analysis of CpG Islands and miRNAs binding sites on LncRNAs, MALAT1, and UCA1 was conducted. Also, The ROC curve area (AUC) of MALAT1 was 0.80. The current results suggest that the expression level of MALAT1 could serve as a potential diagnostic biomarker for bipolar patients. Keywords MALAT1 . UCA1 . Long non-coding RNA . Bipolar disorder . Biomarker . Gene expression
Introduction Bipolar disorders (BD) are chronic and recurrent diseases affecting 1% of the adult world population. It is characterized by
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s11011-020-00580-9) contains supplementary material, which is available to authorized users. * Bahar Naghavi Gargari [email protected] 1
Department of Cell and Molecular Biology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University G.C, Tehran, IR, Iran
2
Behavioral Science Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IR, Iran
3
Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IR, Iran
4
School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IR, Iran
5
Department of Basic Sciences, Faculty of Nursing & Midwifery, Shahid Beheshti University of Medical Sciences, IR Tehran, Iran
impairment of cognitive and function, fluctuations of mood and energy, and increased mortality risk resulted from suicide or cardiovascular diseases (Grande et al. 2016; Vieta et al. 2018a). Also, it is considered as one of the main causes of disability in young people (Grande et al. 2016). The diagnosis and treatment of bipolar disorder are very difficult. The ma
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