Thuja occidentalis mediated AuNPs as wound dressing agents for abdominal wound healing in nursing care after surgery
- PDF / 2,111,510 Bytes
- 8 Pages / 595.276 x 790.866 pts Page_size
- 41 Downloads / 177 Views
ORIGINAL ARTICLE
Thuja occidentalis mediated AuNPs as wound dressing agents for abdominal wound healing in nursing care after surgery Yan Dong1 · Xiu‑Mei Hu1 · Yun‑Feng Cao1 · Ya‑Chao Wang1 · Ling‑Zi Li1 · Jian‑Yu Lu1 · Xiu‑Xia Li1 Received: 18 March 2020 / Accepted: 20 May 2020 © King Abdulaziz City for Science and Technology 2020
Abstract Prevention of infection at the surgical area and outbreak of wounds are the most important and at the same time challenging tasks in clinical care. This research investigates the healing effect of laparotomy wounds after the use of bio-fabricated gold nanoparticles (Au NPs) prepared utilizing Thuja occidentalis leaf extract. Ultraviolet–visible (UV–Vis) spectroscopy and X-ray diffraction (X-RD) results confirmed the successful formation of Au NPs. High-resolution Transmission Electron Microscopy (HR-TEM) images confirmed the formation of spherical shaped Au NPs comprising of 30–50 nm particle size. Dermal fibroblasts were subjected to increased concentrations of Au NPs and their effect of cytotoxicity and synthesis of collagen was evaluated. The procedure of celiotomy was conducted on rabbits where the surgical area was exposed topically with either vehicle or once/everyday with Au NPs for about 14 days. Au NPs raised the collagen production from dermal fibroblasts and improved the expression of caspase 3 by exposure to a long period. AuNPs also showed cytotoxic impact with half-maximal inhibitory concentration ( IC50) of 0.16 mg/mL. Regular treatment of surgical area with AuNPs led to higher collagen deposition with reduced microbial load and enhanced healing of wounds. Keywords Au NPs · Abdominal wound healing · Fibroblasts
Introduction Laparotomy is recognized as one among the commonly conducted surgical procedures in medical care. Infection in surgical area as well as wound dehiscence are the important complications of post laparotomy surgery. The incidence rate of infection at the surgical site ranges from 2.5 to 41.9% globally (Mawalla et al. 2011). The possibility of occurring wound dehiscence after laparotomy surgery ranges from 0.25 to 3%, (Hugh 1990; Mokela et al. 1995; Heller et al. 2006) however, such cases sometimes require emergency surgery that often results in fatal and 20% of these patients have reported being dead (Spiliotis et al. 2009). Like many other causes including hematoma, improper usage of the procedure, and suture material, infection at the operational site is also a prominent cause of wound dehiscence post laparotomy. Laparotomy includes making a complete thickness * Xiu‑Xia Li [email protected] 1
Department of General Surgery, Weifang People’s Hospital, No. 151 Guangwen Street, Kuiwen District, Weifang, Shandong 261041, China
injury in the abdomen, which involves the peritoneum to enter the abdominal cavity, rendering the wound more vulnerable to systemic and local infections relative to superficial wounds; in turn, the existence of fat in the abdomen could be an added factor to increased risks of post-laparotomy infection. It is ther
Data Loading...
