TILLING by sequencing (TbyS) reveals mutations in flowering control genes that are associated with altered plant archite
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RESEARCH ARTICLE
TILLING by sequencing (TbyS) reveals mutations in flowering control genes that are associated with altered plant architecture in Mungbean (Vigna radiata (L.) R. Wilczek) Anusheela Varadaraju . Bharathi Raja Ramadoss . Selvakumar Gurunathan . Ramya Balaram . Kulandaivelu Ganesamurthy . Sundaram Ganesh Ram
Received: 11 February 2020 / Accepted: 17 September 2020 Ó Springer Nature B.V. 2020
Abstract Mutation is prerequisite for creating new variation in crop plants. This variation forms the basis for any crop improvement program. In this study, a population of 3774 mungbean M1 mutant plants were generated using 0.7% of ethyl methane sulfonate (EMS). For TILLING by sequencing, 768 M2 families were sequenced for three different candidate genes responsible for altering plant architecture in mungbean. In total, five exon residing mutations were identified, among them three mutations each in GIGANTEA (GI), RAMOSUS (RMS) and TERMINAL FLOWER1 (TFL1) were found to be deleterious and altering the protein function based on SIFT analysis. Through morphological characterization, we Anusheela Varadaraju and Bharathi Raja Ramadoss have contributed equally to this work.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10722-020-01028-w) contains supplementary material, which is available to authorized users. A. Varadaraju (&) S. Gurunathan R. Balaram K. Ganesamurthy S. Ganesh Ram Department of Plant Genetic Resources, Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu 641003, India e-mail: [email protected] B. R. Ramadoss Department of Plant Sciences, College of Agriculture and Bioresources, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK S7N 5A8, Canada
confirmed the potential role of each mutation in altering mungbean plant architecture to develop an ideal plant type to increase the yield potential. Additionally, mutants with desirable traits such as increase in number of branches, and terminal and late flowering were selected from this study and advanced to further generation, as well as we are planning to pyramiding all three mutation in single genetic background that could contribute potentially towards developing high yielding mungbean genotypes with ideal plant type. Keywords TILLING by sequencing Mungbean Mutation Plant architecture
Introduction Mungbean (Vigna radiata (L.) R. Wilczek) is a fastgrowing legume cultivated mostly in South, East and Southeast Asia by small and marginal farmers for its edible seeds and sprouts. Mungbean seeds provide an invaluable source of digestible protein, folate, and iron than any other legumes. The quantity and quality of protein in its grains makes it deserverdly considered as an alternative vegetarian protein source where meat is scanty (Kang et al. 2014). Mungbean can also be processed and used for various food products such as vermicelli, bean thread noodles, cellophane noodles, and fried dal. The
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