Total Thyroidectomy in the Mouse: the Feasibility Study in the Non-thyroidal Tumor Model Expressing Human Sodium/Iodide
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ORIGINAL ARTICLE
Total Thyroidectomy in the Mouse: the Feasibility Study in the Non-thyroidal Tumor Model Expressing Human Sodium/Iodide Symporter Gene Hye-kyung Shim & Seog Gyun Kim & Tae-Sung Kim & Seok-ki Kim & Sang Jin Lee
Received: 30 June 2010 / Accepted: 24 January 2011 / Published online: 15 February 2011 # Korean Society of Nuclear Medicine 2011
Abstract Purpose This study sought to probe the feasibility of performing total thyroidectomy in the mouse using a nonthyroidal hNIS expressing tumor model. Materials and Methods Our thyroidectomy protocol included thorough excision of both lobes and the isthmus. For evaluating the completeness of thyroidectomy, we compared the 99mTcpertechnetate scans taken before and after thyroidectomy. The prostate cancer cell line was subcutaneously inoculated 2 weeks after the thyroidectomy. When the tumor reached 5-10 mm in diameter, Ad5/35-E4PSESE1a-hNIS was injected intratumorally, and 131I scans were performed. The radioiodine uptakes of the neck and the tumor were compared with those of the other regions. Results Total thyroidectomy was performed in 13 mice. Although 38.5% died during or just after thyroidectomy, the others survived in good health for 2 months. Thyroid tissue was completely eliminated using our protocol; the residual uptake of 99mTc-pertechnetate was minimal in the neck area. The neck/background uptake ratio after thyroidectomy was significantly lower than that before thyroidectomy (p
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