Treatment of cartilage defects by Low-intensity pulsed ultrasound in a sheep model
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Treatment of cartilage defects by Low-intensity pulsed ultrasound in a sheep model Parviz Vahedi . Hassan Hosainzadegan . Bala Brazvan . Leila Roshangar . Hajar Shafaei . Ramin Salimnejad
Received: 5 February 2020 / Accepted: 1 November 2020 Ó Springer Nature B.V. 2020
Abstract Aim of this study was to evaluate effects of Low-intensity pulsed ultrasound on repair of articular cartilage defects. Low-intensity pulsed ultrasound (Lipus) can induce the differentiation and activation of chondrocytes. This study was designed to evaluate the effect of Lipus on articular cartilage defects in a sheep. Eight sheep were divided in to two groups. The animals received bilateraly, articular cartilage defects 4 mm in diameter and 2 mm in deep on the patellar groove and experimental groups were
treated with intensity 200 mW/cm2, 20 min/day with low-intensity pulsed ultrasound for 2 month. Then both knee joints underwent surgery for remove of formed tissue sample from defects.The samples were evaluated by Quantitative real-time polymerase chain reaction (qRT-PCR), Safranin-o staining, Immunofluorescence Staining and Morphological characterization. The best and worst sample per group according to Macroscopic and micriscopic scoring were icentified. The results showed that the operated groups with-
P. Vahedi (&) H. Hosainzadegan B. Brazvan L. Roshangar H. Shafaei R. Salimnejad Department of Anatomical Sciences, Maragheh University of Medical Sciences, Maragheh, Iran e-mail: [email protected]
P. Vahedi H. Hosainzadegan B. Brazvan L. Roshangar H. Shafaei R. Salimnejad Department of Anatomical Sciences, Ardabil University of Medical Sciences, Ardabil, Iran
P. Vahedi H. Hosainzadegan B. Brazvan L. Roshangar H. Shafaei R. Salimnejad Department of Basic Sciences, Maragheh University of Medical Sciences, Maragheh, Iran P. Vahedi H. Hosainzadegan B. Brazvan L. Roshangar H. Shafaei R. Salimnejad Department of Basic Sciences, School of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran P. Vahedi H. Hosainzadegan B. Brazvan L. Roshangar H. Shafaei R. Salimnejad Department of Anatomical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
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Lipus-treatment and without-Lipus treatment had considered statistically significant. Gross photography revealed that the defects in experimental groups were filled with proliferative tissue, while in control groups, a thin layer of proliferative tissue was formed in defects. qRT-PCR results showed the expression of coll2, sox9, aggrecan and Osteocalcin in experimental groups. Intense safranin-O staining show the formation cartilage tissue in ultrasound treated group, while loose safranin-o-staining were observed at the control groups. Immunofluorescence staining showed the type 2 Collagen protein expression. We suggest that lowintensity pulsed ultrasound provide the mechanistic basis force for articular cartilage repair and effective
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