Trilobatin ameliorates insulin resistance through IRS-AKT-GLUT4 signaling pathway in C2C12 myotubes and ob/ob mice
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Chinese Medicine Open Access
RESEARCH
Trilobatin ameliorates insulin resistance through IRS‑AKT‑GLUT4 signaling pathway in C2C12 myotubes and ob/ob mice Min Liu1,2, Lujing Wang1,2, Xigan Li1,2, Yucui Wu1,2, Fei Yin1,2* and Jianhui Liu1,2*
Abstract Background: Trilobatin, a natural compound, has been found to exhibit anti-diabetic properties in high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic mice. But up to now no research has been reported on the effect of trilobatin on insulin resistance in peripheral tissues. Herein, we determined the effects of trilobatin on insulin resistance in palmitate-treated C2C12 myotubes and ob/ob mice. Methods: Male ob/ob mice (8-10 weeks) and same background C57BL/6 mice were used to evaluate the role of trilobatin on insulin resistance; protein expression and phosphorylation were measured by western blot; glucose uptake was determined a fluorescent test. Results: Treatment with trilobatin prevented palmitate-induced insulin resistance by enhancing glucose uptake and the phosphorylation of insulin resistance substrate 1 (IRS1) and protein Kinase B, (PKB/AKT), recovered the translocation of GLUT4 from cytoplasm to membrane, but preincubation with LY294002, an inhibitor of PI3K, blocked the effects of trilobatin on glucose uptake and the distribution of GLUT4 in C2C12 myotubes. Furthermore, administration with trilobatin for 4 weeks significantly improved insulin resistance by decreasing fasting blood glucose and insulin in serum, enhancing the phosphorylation of IRS1 and AKT, and recovering the expression and translocation of GLUT4 in ob/ob mice. Conclusions: IRS-AKT-GLUT4 signaling pathway might be involved in trilobatin ameliorating insulin resistance in skeletal muscle of obese animal models. Keywords: Glucose transporter 4 (GLUT4), Insulin receptor substrate 1 (IRS1), Insulin resistance, Protein kinase B (PKB/ AKT), Trilobatin Highlights Trilobatin improves insulin resistance in skeletal muscle. Trilobatin enhances glucose uptake in insulin-resistant C2C12 myotubes.
*Correspondence: [email protected]; [email protected] 1 Chongqing Key Lab of Medicinal Chemistry & Molecular Pharmacology, Chongqing University of Technology, Hongguang Road 69, Ba’nan District, Chongqing 400054, China Full list of author information is available at the end of the article
IRS-AKT-GLUT4 signaling pathway is involved in trilobatin improving insulin resistance.
Background Increasing evidence indicates that obesity and type 2 diabetes has elevated over years in both developing and developed country, which are closely associated with insulin resistance, a pathophysiological condition characterized by an impaired insulin action in insulin-sensitizing tissues including liver, adipose tissue and skeletal muscle [1, 2]. Skeletal muscle accounts for 40% of body
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