TRPV4 and TRPM8 as putative targets for chronic low back pain alleviation
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ION CHANNELS, RECEPTORS AND TRANSPORTERS
TRPV4 and TRPM8 as putative targets for chronic low back pain alleviation Stefania Fozzato 1 & Nicolò Baranzini 2 & Elena Bossi 2,3 Paola Campomenosi 2 & Michele Francesco Surace 2,4
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Raffaella Cinquetti 2
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Annalisa Grimaldi 2
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Received: 25 August 2020 / Revised: 25 August 2020 / Accepted: 2 September 2020 # The Author(s) 2020
Abstract The purpose of this study is to investigate the presence of nervous fibers and expression of TRP channels in samples harvested during decompressive/fusion spine surgeries from patients affected by chronic low back pain (CLBP). The aim was to understand if members of this family of receptors played a role in detection and processing of painful stimuli, to eventually define them as potential targets for CLBP alleviation. Expression of transient receptor potential (TRP) channels (A1, V1, V2, V4, and M8) was evaluated in samples from different periarticular sites of 6 patients affected by CLBP, at both protein and transcript levels. The capsular connective pathological tissue appeared infiltrated by sensitive unmyelinated nervous fibers. An increase in TRP channel mRNAs and proteins was observed in the pathological capsule compared with tissues collected from the nonsymptomatic area in five of the six analyzed patients, independently by the location and number of affected sites. In particular, TRPV4 and TRPM8 were consistently upregulated in pathological tissues. Interestingly, the only patient showing a different pattern of expression also had a different clinical history. TRPV4 and TRPM8 channels may play a role in CLBP and warrant further investigations as possible therapeutic targets. Keywords Transient receptor potential . Chronic low back pain . TRPV4 . TRPM8 . Expression . Targets
Introduction Chronic low back pain (CLBP) is a painful condition arising from spinal structures such as bones, joints, muscles, tendons,
ligaments, and intervertebral disks due to traumatic, degenerative, or inflammatory diseases. CLBP is a highly prevalent condition associated with disability, work absenteeism, and huge healthcare costs [36].
Paola Campomenosi and Michele Francesco Surace share last authorship. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00424-020-02460-8) contains supplementary material, which is available to authorized users. * Elena Bossi [email protected] Stefania Fozzato [email protected] Nicolò Baranzini [email protected]
Michele Francesco Surace [email protected] 1
Department of Medicine and Surgery, University of Insubria, Varese, Italy
2
Department of Biotechnology and Life Sciences, University of Insubria, Via Dunant 3, 21100 Varese, VA, Italy
3
Center for Neuroscience Research, University of Insubria, Via Dunant 3, 21100 Varese, VA, Italy
4
Interdisciplinary Research Centre for Pathology and Surgery of the Musculoskeletal System, University of Insubria, Varese, Italy
Raffaella Cinquetti [email protected] Annalisa Gr
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