Tumor promoting effects of circRNA_001287 on renal cell carcinoma through miR-144-targeted CEP55
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(2020) 39:269
RESEARCH
Open Access
Tumor promoting effects of circRNA_001287 on renal cell carcinoma through miR-144-targeted CEP55 Jiafu Feng1*†, Yongcan Guo2†, Yuanmeng Li3†, Jiawei Zeng1, Yaodong Wang4, Yuwei Yang1, Gang Xie5 and Qian Feng6
Abstract Background: Renal cell carcinoma (RCC) is a common urological cancer. circular RNAs (circRNAs) is involved in the development of various types of cancers. However, the roles and underlying mechanisms of circRNAs in RCC are not fully elucidated. Herein, we aimed to examine the potential effect of circ_001287 on RCC progression. Materials and Methods: Microarray-based gene expression profiling of RCC was initially employed in order to identify differentially expressed genes. Next, the expression of circ_001287 was examined, and the cell line with the highest circ_001287 expression was selected for subsequent investigation. The interaction among circ_001287, miR144, and CEP55 was identified by conducting luciferase reporter assay, RNA-pull down, RIP, RT-qPCR and FISH. The effect of circ_001287 on proliferative, invasive and migratory capacities as well as tumorigenicity of transfected cells in mice was examined using gain- and loss-of-function experiments. Results: circ_001287 and CEP55 were highly expressed while miR-144 was decreased in RCC tissues and cell lines. circ_001287 can up-regulate CEP55 by binding to miR-144, which resulted in increased proliferative, invasive and migratory capacities and tumor growth in vivo. In addition, down-regulation of miR-144 was also observed to promote these biological activities. Conclusions: Overall, these results elucidate a new mechanism for circ_001287 in RCC development and provide a potential therapeutic target for RCC patients. Keywords: circ_001287, miR-144, CEP55, Renal cell carcinoma, Proliferation, Migration, Invasion, Apoptosis
Background Renal cell carcinoma (RCC) ranks the third leading cause of death in humans suffering urological tumors [1]. The incidence rate between male and female is 3: 1 and the prevalence increases along with age and reaches the peak at the age between 60 and 70 [2]. * Correspondence: [email protected] † Jiafu Feng, Yongcan Guo and Yuanmeng Li contributed equally to this work and should be considered the first authors. 1 Department of Clinical Laboratory, Mianyang Central Hospital, No. 12, Changjia Lane, Jingzhong Street, Fucheng District, Sichuan Province 621000 Mianyang, PR China Full list of author information is available at the end of the article
RCC is a disorder that results from the renal epithelium and afflicts more than 90% of cancers in the kidney [3]. Patients suffering localized RCC can be treated with nephrectomy. Approximate one quarter of the patients have relapses in distant sites and the distant metastases often occur in the lymph nodes, lungs, livers, bones and brains [4]. The survival rate for patients with maligant RCC is reported to be extremely low [5]. The von Hippel Lindau (VHL) is a typical tumor suppressor gene, and most RCC have VHL gene abnormalities inclu
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