Tumor suppressor TSLC1 is implicated in cell proliferation, invasion and apoptosis in laryngeal squamous cell carcinoma
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RESEARCH ARTICLE
Tumor suppressor TSLC1 is implicated in cell proliferation, invasion and apoptosis in laryngeal squamous cell carcinoma by regulating Akt signaling pathway Baocai Lu & Wenyu Di & Huimin Wang & Huimin Ma & Jinsong Li & Qunmei Zhang
Received: 19 June 2012 / Accepted: 4 July 2012 / Published online: 18 July 2012 # International Society of Oncology and BioMarkers (ISOBM) 2012
Abstract Overwhelming evidence has demonstrated that TSLC1 (tumor suppressor in lung cancer 1), a novel tumor suppressor, is crucially implicated in various biological processes including progression, proliferation and apoptosis during tumorigenesis. However, the exact functions and molecular details of TSLC1 in laryngeal cancer remain illdefined. Here, the expression of TSLC1 in laryngeal squamous cell carcinoma (LSCC) tissues and cells was detected, and the biological roles of TSLC1 in LSCC cells were investigated. The results showed that expressions of TSLC1 mRNA and protein were significantly reduced in LSCC tissues with low expression in 18 of 85 (21.18 %) and 16 of 85 (18.82 %), respectively. Additionally, statistical analysis revealed a significant correlation of TSLC1 expression with TNM staging and lymph node metastases (P0.05). Elevation of TSLC1 level inhibited cell proliferation, reduced cell invasion in vitro and induced cell apoptosis in Hep-2 cells, most importantly, TSLC1 upregulation decreased the level of pAkt, but not changed the level of total Akt in Hep-2 cells. Stepwise B. Lu (*) : H. Wang : H. Ma : J. Li Department of Otorhinolaryngology, First Affiliated Hospital of Xinxiang Medical College, No. 88 JianKang Road, Weihui, Henan 453100, China e-mail: [email protected] W. Di Department of Pathology, First Affiliated Hospital of Xinxiang Medical College, Weihui, Henan 453100, China Q. Zhang Department of Blood Transfusion, First Affiliated Hospital of Xinxiang Medical College, Weihui, Henan 453100, China
investigations demonstrated that overexpression of TSLC1 in Hep-2 cells increased caspase-3 activity and expressions of bax and p21 proteins but decreased the levels of bcl-2, MMP2 and MMP-9 proteins. These data suggest that TSLC1 may exert essential roles in the progression and development of LSCC, and thus TSLC1 may be a potential molecular target for LSCC treatment. Keywords Laryngeal squamous cell carcinoma . Tumor suppressor in lung cancer 1 . Cell proliferation . Cell invasion . Cell apoptosis . Akt signaling pathway
Introduction Head and neck squamous cell carcinoma (HNSCC) ranks as the sixth most common cancer worldwide [1]. Laryngeal carcinoma is one of the most frequently occurring tumors of HNSCC, with 159,000 new cases and 90,000 deaths annually reported worldwide [1, 2]. More than 90 % of laryngeal cancer has been pathologically diagnosed as laryngeal squamous cell carcinoma (LSCC) [3]. LSCC at early stage can be treated either by beam radiation therapy, or a combination of surgery and irradiation, with an overall 5-year survival of >65 % [4, 5]. Nevertheless, most cases are diagnosed at an adva
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