Ultrasensitive Isothermal Detection of Protein Analytes Using Rolling Circle Amplification in Microscale Platforms
The detection of low-abundance analytes requires an accurate, highly sensitive, and robust bioassay platform. Rolling Circle Amplification (RCA) has been increasingly utilized in isothermal diagnostics of nucleic acids and other biomarkers of various dise
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Rolling Circle Amplification (RCA) Toward New Clinical Diagnostics and Therapeutics
Rolling Circle Amplification (RCA)
Vadim V. Demidov Editor
Rolling Circle Amplification (RCA) Toward New Clinical Diagnostics and Therapeutics
Editor Vadim V. Demidov Global Prior Art Inc. Boston, MA, USA
ISBN 978-3-319-42224-4 ISBN 978-3-319-42226-8 DOI 10.1007/978-3-319-42226-8
(eBook)
Library of Congress Control Number: 2016955094 © Springer International Publishing Switzerland 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Springer imprint is published by Springer Nature The registered company is Springer International Publishing AG The registered company address is: Gewerbestrasse 11, 6330 Cham, Switzerland
To Andrew Fire, Eric Kool, Paul Lizardi, David Zhang, Ulf Landegren, Jerry Ruth, David Driver and Jeff Auerbach, who were the first few to realize the great potential of rolling replication of small DNA circles, and who introduced this innovative methodology to bioresearchers in the 1990s, therefore laying the foundations of current RCA technology
Advance Praise for Rolling Circle Amplification (RCA)
“The ability to amplify nucleic acids in the test tube has revolutionized nearly every aspect of research and diagnostics based on genetic material. For the past few decades the polymerase chain reaction (PCR) has been the dominant amplification method. However because PCR requires thermal cycling it is difficult to adapt to some important experimental applications. A few different approaches to circumvent the need for thermal cycling have been developed but most of these have failed to gain widespread adoption. The exception is rolling circle amplification (RCA) which has matured into a widely used powerful alternative to PCR for many applications. This book provides a badly needed compendium of innovative RCA methods and applications. It should help further increase the community of scientists that have employed RCA in research and diagnostic p
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