Ultrasound-assisted extraction and in vitro simulated digestion of Porphyra haitanensis proteins exhibiting antioxidativ
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ORIGINAL PAPER
Ultrasound‑assisted extraction and in vitro simulated digestion of Porphyra haitanensis proteins exhibiting antioxidative and α‑glucosidase inhibitory activity Lin Wen1 · Siwen Tan1 · Limin Zeng1 · Zhufeng Wang2 · Xingyao Ke3 · Zheqing Zhang4 · Huanwen Tang1 · Honghui Guo1 · Enqin Xia1 Received: 9 January 2020 / Accepted: 12 July 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Peptides from natural materials have been reported owning potential for regulating oxidative stress and hyperglycemia for human body. In this study, peptides from a favorite food, Porphyra haitanensis, were prepared by consecutive procedures including ultrasound-assisted extraction, in vitro simulated gastrointestinal digestion and fraction. Subsequently, their relevant bioactivities were investigated by several in vitro methods, such as 1,1-diphenyl-2-pycrylhydrazyl (DPPH) radical scavenging, ferric reducing antioxidant power (FRAP), and α-glucosidase enzyme inhibition assays. By single factor design experiment, the highest yield of crude protein was 3.42% extracted by alkaline solution for 20 min sonication at 35 °C. After digestion, peptides were mainly distributed in F2–F11 fractions by sephadex G-25, and the peak value was found in F5. The significant activities in three assays were displayed in F3–F7. As calculated by the polynomial curve fitting, a significantly positive correlation was found between the peptide contents and the DPPH radical scavenging rate, the FRAP value, and the α-glucosidase enzyme inhibitory rate (R2 = 0.8490, R2 = 0.7579, R2 = 0.9701, respectively). The results showed that these peptides possessed promising antioxidant activity and inhibitory activity on α-glucosidase. Therefore, peptides derived from P. haitanensis have potential anti-diabetic applications in pharmaceutical and functional food industries. Keywords Porphyra haitanensis · Ultrasound-assisted extraction · In vitro simulated digestion peptide · Antioxidant activity · Alpha-glucosidase inhibition activity
Introduction
* Enqin Xia [email protected] 1
Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, No. 1 Newtown Road, Dongguan 523808, China
2
State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, No. 151 Yan Jiang Road, Guangzhou 510120, China
3
Department of Epidemiology, School of Medicine, Jinan University, No. 601 Huangpu Road West, Guangzhou, Guangdong 510632, China
4
Department of Nutrition and Food Hygiene, School of Public Health, Southern Medical University, No. 1C‑1046 Shatai Southern Road, Guangzhou 510515, China
Nowadays, type 2 diabetes mellitus (T2DM) has been one of the common diseases that affect human health globally and has been widely concerned, ever-increasing diabetes population caused heavy economic burden [1]. Many drugs have been developed and applied to deal with the diabetics, such as rosiglitazone
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