Validation of marker-assisted selection in soybean breeding program for pod shattering resistance
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Validation of marker-assisted selection in soybean breeding program for pod shattering resistance Ji-Min Kim . Kyung-Hye Kim . Jiyeong Jung . Beom Kyu Kang . Juseok Lee . Bo-Keun Ha . Sungtaeg Kang
Received: 6 July 2020 / Accepted: 26 September 2020 Ó Springer Nature B.V. 2020
Abstract Significant yield losses are caused by pod shattering during the harvest of domesticated crop plants. The phenotype evaluation of pod shattering requires fully grown plants, and it is a labor-intensive and time-consuming procedure. The purpose of this study was to test the applicability of previously identified KSS-SNP5 SNP marker on chromosome 16 in the marker assisted selection (MAS) system for pod shattering in soybean breeding programs. For this research, TaqMan SNP assay was successfully developed to detect the A/G allele of the KSS-SNP5 marker in the breeding materials, including two breeding
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10681-020-02703-w) contains supplementary material, which is available to authorized users. J.-M. Kim K.-H. Kim J. Jung S. Kang (&) Department of Crop Science and Biotechnology, Dankook University, Cheonan 31116, Korea e-mail: [email protected] B. K. Kang National Institute of Crop Science, Rural Development Administration, Miryang 50424, Korea J. Lee Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 28116, Korea B.-K. Ha Department of Applied Plant Science, Chonnam National University, Gwangju 61186, Korea
stages, F2:3 populations, and yield trial stage breeding lines. The MAS prediction accuracy was calculated by comparing the genotyping data from the TaqMan assay with the phenotype data from the dry-oven method. The total MAS prediction accuracy was 92.5% and 96.2% in two F2:3 populations and breeding lines, respectively and 85% and 96% accuracy were shown in severe conditions. In this study, we confirmed that the KSS-SNP5 TaqMan probe tightly linked to pod shattering is effective to select a resistant line. Based on these results, we propose that the marker tightly linked to pod shattering can be of valuable use in soybean breeding program for the development of pod shattering-resistant cultivars. Keywords Pod shattering Single-nucleotide polymorphism Marker-assisted selection Glycine max Selection accuracy Breeding
Introduction Plant pod shattering is an important procedure for spreading progenies, which is associated with the translocation abilities of wild/weed species. However, pod shattering is one of the major causes of yield loss in legume and crucifer crops, and it is controlled by many physiological and biochemical processes (Dong et al. 2014). Abscission layers are located at the
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binding sites of the pod walls. During the seed maturation process, a dehiscing force is accumulated in the abscission layer. When this dehiscing force becomes greater than the binding strength of the pod walls, pod shatterin
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