Vitrification of camel oocytes transiently impacts mitochondrial functions without affecting the developmental potential
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SHORT RESEARCH AND DISCUSSION ARTICLE
Vitrification of camel oocytes transiently impacts mitochondrial functions without affecting the developmental potential after intracytoplasmic sperm injection and parthenogenetic activation Islam M. Saadeldin 1,2 & Fariba Moulavi 3 & Ayman Abdel-Aziz Swelum 1,4 & Sokhangouy Saiede Khorshid 3 & Hossini-Fahraji Hamid 3 & Sayyed Morteza Hosseini 3 Received: 14 August 2020 / Accepted: 30 September 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Oocyte vitrification preserves the female genetic resources of elite dromedary camels. In the current study, we aimed to explore the effects of vitrification of camel oocytes on mitochondrial activity, redox stress, and expression of genes related to mitochondrial function, apoptosis, pluripotency, and cytoskeleton. Moreover, we investigated developmental competence of vitrified oocytes after parthenogenetic activation. Oocytes vitrified with the Cryotop method were compared with the fresh oocytes. Our results showed that vitrification led to increased ROS production in oocytes as evidenced by an increase in the DCFDHA fluorescence intensity, and lower mitochondrial activity. At the molecular level, vitrification reduced mRNA expression of many genes, including those related to mitochondrial function (TFAM, MT-CO1, MFN1, ATP1A1, NRF1), pluripotency (SOX2 and POU5F1), and apoptosis (p53 and BAX). In contrast, expression of KLF4 and cytoskeleton-related genes (ACTB and KRT8) was not affected. However, we found no difference in the rates of oocyte survival, cleavage, and blastocyst development, and blastocyst hatching between fresh and vitrified oocytes after warming. Our results indicate that although vitrification of camel metaphase II (MII) oocytes adversely affected mitochondrial functions, the effect was transient without compromising the developmental potential of the oocytes after parthenogenetic activation. Keywords Vitrification . Oocytes . Real-time polymerase chain reaction . Reactive oxygen species . Mitochondria . Camel
Highlights • The effects of vitrification on camel MII oocytes have been studied. • Vitrification adversely affected mitochondrial protein and mRNA transcripts expression. • The adverse effect was transient and did not affect the developmental potential of oocytes after ICSI and parthenogenetic activation. Responsible editor: Mohamed M. Abdel-Daim * Islam M. Saadeldin [email protected]
2
Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt
* Sayyed Morteza Hosseini [email protected]
3
Department of Embryology, Camel Advanced Reproductive Technologies Centre, Government of Dubai, Dubai, United Arab Emirates
1
4
Department of Theriogenology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt
Department of Animal Production, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi Arabia
Environ Sci Pollut Res
Introduction Preservation of female genetic pool can be achiev
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