Zygote arrest 1 gene in pig, cattle and human: evidence of different transcript variants in male and female germ cells
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Zygote arrest 1 gene in pig, cattle and human: evidence of different transcript variants in male and female germ cells Svetlana Uzbekova*1, Monica Roy-Sabau1, Rozenn Dalbiès-Tran1, Christine Perreau1, Pascal Papillier1, Florence Mompart3, Aurore Thelie1, Sophie Pennetier1, Juliette Cognie1, Veronique Cadoret1,2, Dominique Royere2, Philippe Monget1 and Pascal Mermillod1 Address: 1Physiologie de la Reproduction et des Comportements, UMR 6175 Institut National de la Recherche Agronomique/Centre National de la Recherche Scientifique, Université François Rabelais de Tours, Haras Nationaux, 37380 Nouzilly, France, 2Service de Médecine et Biologie de la Reproduction, UMR 6175, Centre Hospitalier Universitaire Bretonneau, 37044 Tours, France and 3Laboratoire de Génétique Cellulaire, INRA, Chemin de Borde-Rouge – Auzeville, BP 52627 31326 Castanet-Tolosan Cedex, France Email: Svetlana Uzbekova* - [email protected]; Monica Roy-Sabau - [email protected]; Rozenn DalbièsTran - [email protected]; Christine Perreau - [email protected]; Pascal Papillier - [email protected]; Florence Mompart - [email protected]; Aurore Thelie - [email protected]; Sophie Pennetier - [email protected]; Juliette Cognie - [email protected]; Veronique Cadoret - [email protected]; Dominique Royere - [email protected]; Philippe Monget - [email protected]; Pascal Mermillod - [email protected] * Corresponding author
Published: 21 March 2006 Reproductive Biology and Endocrinology2006, 4:12
doi:10.1186/1477-7827-4-12
Received: 06 December 2005 Accepted: 21 March 2006
This article is available from: http://www.rbej.com/content/4/1/12 © 2006Uzbekova et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract Background: Zygote arrest 1 (ZAR1) is one of the few known oocyte-specific maternal-effect genes essential for the beginning of embryo development discovered in mice. This gene is evolutionary conserved in vertebrates and ZAR1 protein is characterized by the presence of atypical plant homeobox zing finger domain, suggesting its role in transcription regulation. This work was aimed at the study of this gene, which could be one of the key regulators of successful preimplantation development of domestic animals, in pig and cattle, as compared with human. Methods: Screenings of somatic cell hybrid panels and in silico research were performed to characterize ZAR1 chromosome localization and sequences. Rapid amplification of cDNA ends was used to obtain full-length cDNAs. Spatio-temporal mRNA expression patterns were studied using Northern blot, reverse transcription coupled to polymerase chain reaction and in situ hybridization. Results: We demonstrated that ZAR1 is a single copy gene, positioned on chromos
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