1,25(OH) 2 D 3 -Mediated Amelioration of Aortic Injury in Streptozotocin-Induced Diabetic Rats
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1,25(OH)2D3-Mediated Amelioration of Aortic Injury in Streptozotocin-Induced Diabetic Rats Fengao Li,1 Ping Liu,2 Xin Zhang,1 Qiuzi Zhang,1 Shaofang Tang,1 Mei Zhu,1 and Mingcai Qiu1,3
Abstract—In this study, a single tail vein injection of streptozotocin (STZ)-induced rat model was employed to study the effects of 1,25(OH)2D3 supplementation, the active form of vitamin D, on diabetes-induced aortic injury. Aortas from different groups were assessed for histopathology, tolllike receptor 4 (TLR4), myeloid differentiation primary response gene 88 (MyD88), and nuclear factor-kappa B (NF-κB) p65 expression by hematoxylin and eosin staining, immunohistochemistry staining, reverse transcription polymerase chain reaction, and Western blot analysis. High-dose 1,25(OH)2D3 (0.3 μg/kg/day) significantly prevented diabetes-induced aortic pathological changes and collagen deposition and decreased the expression of TLR4, MyD88, and NF-κB at both mRNA and protein levels in the aorta of STZ-induced diabetic rats (P
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