A competitive immunoassay based on engineered magnetic/fluorescent nanoparticles and biolayer interferometry-based assay
- PDF / 1,782,684 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 2 Downloads / 188 Views
ORIGINAL PAPER
A competitive immunoassay based on engineered magnetic/fluorescent nanoparticles and biolayer interferometry-based assay for T-2 toxin determination Yafan Li 1 & Li Xu 1
&
Xiaochen Fu 1 & Hongxiang Liu 1 & Guo-Bin Ding 2 & Lin Song Li 3 & Jinming Zhu 4 & Yi Guo 1
Received: 2 April 2020 / Accepted: 17 August 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract For the first time a competitive immunoassay was developed by employing T-2 antibody-functionalized magnetite nanoparticles and T-2 toxin-conjugated fluorescent quantum dots (QDs). Free T-2 and the T-2-modified QDs compete for binding to antibodymodified magnetic beads; the magnetic beads collected by magnetic separation were subjected to fluorescence intensity analysis (with excitation/emission wavelengths at 460/616 nm). This competitive immunoassay for T-2 toxin determination was applied both in a microcentrifuge tube and on a 96-well plate. The dynamic range of the immunoassay is 1–100 ng mL−1, the limit of detection (LOD) is 0.1 ng mL−1, and determination was completed in about 40 min and 30 min in the microcentrifuge tube and 96-well plate, respectively. Moreover, the biolayer interferometry (BLI) technique was employed for T-2 determination for the first time, in which the conjugate of T-2 toxin and bovine serum albumin (BSA) was immobilized on the sensors before detection. Its average recovery of T-2 toxin from barley sample ranged from 82.00 to 123.33%, and the relative standard deviation (RSD) was between 9.42 and 15.73%. The LOD of the BLI-based assay is 5 ng mL−1, and it only takes 10 min to finish the determination. Keywords Competitive immunoassay . Magnetic polystyrene nanospheres . Quantum dots . Biolayer interferometry . T-2 toxin determination
Introduction Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04493-3) contains supplementary material, which is available to authorized users. * Li Xu [email protected] * Guo-Bin Ding [email protected] 1
Key Laboratory for Molecular Enzymology and Engineering, the Ministry of Education, National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun 130012, People’s Republic of China
2
Institute of Biotechnology, The Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, People’s Republic of China
3
Key Laboratory for Special Functional Materials, the Ministry of Education, Henan University, Kaifeng 475004, People’s Republic of China
4
China-Japan Union Hospital of Jilin University, Changchun 130033, People’s Republic of China
As one of the most prevalent type A trichothecenes, T-2 toxin (T-2) is produced by several Fusarium species, such as F. acuinatum, F. poae, and F. soprotrichioides, which can be found in various cereal grains [1]. Because of its 12,13epoxy ring, T-2 is considered as the most acutely toxic trichothecene. And it can cause alimentary toxicity and immunotoxicity by inhibit
Data Loading...
