A new spectrophotometric method for the determination of glyphosate: statistical optimization and application in biodegr
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ORIGINAL PAPER
A new spectrophotometric method for the determination of glyphosate: statistical optimization and application in biodegradation studies V. K. Bhatt1 · B. D. Iyer1 Received: 28 April 2020 / Revised: 10 August 2020 / Accepted: 14 August 2020 © Islamic Azad University (IAU) 2020
Abstract Glyphosate (N-phosphonomethylglycine) is extensively used as herbicide against annual and perennial weeds. In the present study, we report a new colorimetric method for the estimation of glyphosate which is based on formation of copper dithiocarbamic acid complex from glyphosate. Glyphosate formed yellow colored complex (λmax = 372 nm) when it was reacted with carbon disulfide ( CS2) and copper(II)nitrate in alkaline medium. Using Box–Behnken design (BBD), glyphosate, C S 2, copper(II) nitrate, and reaction time were found to influence the absorbance at 372 nm significantly (p
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