A Novel Composite and Suspended Nanofibrous Scaffold for Skin Tissue Engineering
Electrospinning is a technique for creating continuous nanofibrous networks that can architecturally be similar to the structure of extracellular matrix (ECM). In this work, a kind of PLGA/silk fibroin composite electrospinning nanofibrous scaffolds is pr
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Center of Robotics and Micro System & Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, Suzhou 215021, People’s Republic of China 2College of Electronic Information Engineering, Suzhou Vocational University, Suzhou 215104, People’s Republic of China 3Institute of Clinical Immunology, The First Affiliated Hospital of Soochow University, Suzhou 215006, People’s Republic of China
Abstract—Electrospinning is a technique for creating continuous nanofibrous networks that can architecturally be similar to the structure of extracellular matrix (ECM). In this work, a kind of PLGA/silk fibroin composite electrospinning nanofibrous scaffolds is proposed for the growth of skin cells in combination with the good mechanical properties with not easy to deformation of silk fibroin and the self-floating of PLGA. The SEM experiment of the silk fibroin/PLGA composite nanofibers indicated that the prepared nanofibers are smooth, uniform in size and have good porosity. The floating experiment is shown that this scaffold is able to suspend in water and is also able to suspend in cell culture medium, hence, the skin cells seeded on the scaffold are exposed to air as required in skin tissue engineering. The wrinkled contrast experiment and the degradation experiment are verified the usefulness of this scaffold. Keywords—Composite nanofibrous scaffold, silk fibroin, poly(lactic-co-glycolic acid) (PLGA), electrostatic spinning, skin tissue engineering.
I. INTRODUCTION
Skin represents approximately one-tenth of the body mass and is necessary for animal survival [1−4]. In the past decades, many skin substitutes, such as xenografts, autografts, and allografts, have been used to treat burns or other skin defects [5−7]. However, because of factors, such as immune response, these skin substitutes are not yet able to fully meet the requirements of skin recovery [8]. Hence, tissue engineering has been under intense development and has shown significant promise in creating skin tissue substitutes [9−12]. One challenge in tissue engineering is the design of reproducible three-dimensional (3D) scaffolds that can mimic the structure and biological functions of the natural extracellular matrix (ECM), for guiding cellular migrationproviding mechanical support, and regulating cellular activities [13]. Compared to other methods for the fabrication of nanofibrous scaffolds, such as meltblowing [14], drawing, phase-separation and self-assembly, electrospinning is a simple and efficient method for creating continuous nanofibres that present unique physical, chemical, electrical, magnetic and optical properties[15]. © Springer Nature Singapore Pte Ltd. 2018 H. Eskola et al. (eds.), EMBEC & NBC 2017, IFMBE Proceedings 65, DOI: 10.1007/978-981-10-5122-7_1
The method of cultivating skin cells is usually the cells planted on collagen [16]. The skin cells in the culture are often required to contact with the air to imitate the real environment of skin cells, so a cloth with a small hole mesh structure is often used to ca
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