Skin Tissue Engineering Methods and Protocols
This volume provides protocols describing the isolation and culture of diverse cell types stemming from the skin and the use of these cells and cell constructs for wound healing, bioengineering applications, and translational medicine purposes. The book i
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Sophie Böttcher-Haberzeth Thomas Biedermann Editors
Skin Tissue Engineering Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Skin Tissue Engineering Methods and Protocols
Edited by
Sophie Böttcher-Haberzeth and Thomas Biedermann University Children's Hospital Zurich, Zurich, Switzerland
Editors Sophie Bo¨ttcher-Haberzeth University Children’s Hospital Zurich Zurich, Switzerland
Thomas Biedermann University Children’s Hospital Zurich Zurich, Switzerland
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-9472-4 ISBN 978-1-4939-9473-1 (eBook) https://doi.org/10.1007/978-1-4939-9473-1 © Springer Science+Business Media, LLC, part of Springer Nature 2019 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. This Humana imprint is published by the registered company Springer Science+Business Media, LLC, part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Regenerative medicine and especially skin tissue engineering approaches are considered to be the most innovative advancements to the treatment of skin conditions and large wounds originating from traumas, burns, or other devastating skin diseases. The aim of this Methods in Molecular Biology volume is to provide protocols describing the isolation and culture of diverse cell types stemming from the skin and the use of these cells and cell constructs for wound healing, bioengineering applications, and translational medicine purposes. The chapters are divided into three sections describing the isolation and culture of diverse skin cells, managing these cells within co-culture systems and skin models, as well as using these s
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