A Practical Guide to Frozen Section Technique
A Practical Guide to Frozen Section Technique offers an easy to learn approach to frozen section technique in the form of a highly illustrated handbook intended for onsite use in the laboratory. The book begins with a novel, clearly delineated, step
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Stephen R. Peters Editor
A Practical Guide to Frozen Section Technique
Editor Stephen R. Peters University of Medicine and Dentistry of New Jersey New Jersey Medical School Newark, NJ USA [email protected]
ISBN 978-1-4419-1233-6 e-ISBN 978-1-4419-1234-3 DOI 10.1007/978-1-4419-1234-3 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2009933112 © Springer Science+Business Media, LLC 2010 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper Springer is part of Springer Science+Business Media (www.springer.com)
Preface
Frozen section technique is a valuable tool used to rapidly prepare slides from tissue for microscopic interpretation. Frozen section technique is used in a myriad of clinical and research settings. In surgical pathology, frozen sections are routinely used for rapid intra-operative diagnosis, providing guidance for our surgical colleagues. In Mohs Micrographic Surgery, the surgeon relies entirely on the frozen sections to determine the extent of the excision needed to eradicate a skin tumor. Numerous research applications rely on the frozen section technique to prepare microscopic slides utilizing a host of sophisticated morphologic, immunohistochemical and molecular methods. Preparation of frozen section slides is a complex technical process requiring development of refined technical skills, as well as an understanding of the histology, microanatomy and pathology of the tissues being examined. Whether used for intra-operative consultation or in research, the results will hinge on our ability to achieve a high quality preparation. The training in frozen section can vary considerably among the various subsets of practitioners. The subject is part of the curriculum in formal histology and pathologist assistant programs although much of the hands on technique is passed along at the work bench. Likewise in many pathology residency programs and research applications, training is accomplished entirely on the job sometimes with little discussion of the myriad of variables and difficulties the operator will experience along the way. I like many pathology residents received training on the job with little more than a brief introduction to the operation of the cryostat, simple face up embedding, and to cut frozen sections using a brush. My teacher was a resident in his third year of
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