A rapid and sensitive method to detect SARS-CoV-2 virus using targeted-mass spectrometry
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ORIGINAL ARTICLE
A rapid and sensitive method to detect SARS‑CoV‑2 virus using targeted‑mass spectrometry Praveen Singh1,2 · Rahul Chakraborty1,2 · Robin Marwal3 · V. S. Radhakrishan3 · Akash Kumar Bhaskar1,2 · Himanshu Vashisht3 · Mahesh S. Dhar3 · Shalini Pradhan1 · Gyan Ranjan1,2 · Mohamed Imran1,2 · Anurag Raj1,2 · Uma Sharma3 · Priyanka Singh3 · Hemlata Lall3 · Meena Dutta3 · Parth Garg4 · Arjun Ray4 · Debasis Dash1,2 · Sridhar Sivasubbu1,2 · Hema Gogia3 · Preeti Madan3 · Sandhya Kabra3 · Sujeet K. Singh3 · Anurag Agrawal1,2 · Partha Rakshit3 · Pramod Kumar3 · Shantanu Sengupta1,2 Received: 29 July 2020 / Revised: 18 August 2020 / Accepted: 24 August 2020 © Springer Nature Singapore Pte Ltd. 2020
Abstract In the last few months, there has been a global catastrophic outbreak of severe acute respiratory syndrome disease caused by the novel coronavirus SARS-CoV-2 affecting millions of people worldwide. Early diagnosis and isolation are key to contain the rapid spread of the virus. Towards this goal, we report a simple, sensitive and rapid method to detect the virus using a targeted mass spectrometric approach, which can directly detect the presence of virus from naso-oropharyngeal swabs. Using a multiple reaction monitoring we can detect the presence of two peptides specific to SARS-CoV-2 in a 2.3 min gradient run with 100% specificity and 90.5% sensitivity when compared to RT-PCR. Importantly, we further show that these peptides could be detected even in the patients who have recovered from the symptoms and have tested negative for the virus by RT-PCR highlighting the sensitivity of the technique. This method has the translational potential of in terms of the rapid diagnostics of symptomatic and asymptomatic COVID-19 and can augment current methods available for diagnosis of SARS-CoV-2. Keywords SARS-CoV-2 · COVID-19 · LC-MS/MS · Proteomics · MRM · Naso-oropharyngeal swab Praveen Singh, Rahul Chakraborty, Robin Marwal, Radhakrishan have contributed equally. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s42485-020-00044-9) contains supplementary material, which is available to authorized users. * Partha Rakshit [email protected] * Pramod Kumar [email protected] * Shantanu Sengupta [email protected] 1
CSIR-Institute of Genomics and Integrative Biology, Mathura Road, New Delhi 110025, India
2
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India
3
National Center for Disease Control, New Delhi 110054, India
4
Department of Computational Biology, Indraprastha Institute of Information Technology, Okhla Industrial Estate, Phase III, New Delhi 110020, India
Introduction The world is in the midst of a pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). More than 15 million individuals from 200 countries have been infected with this virus, which resulted in over 600,000 deaths (https://www.worldometers.info/coronavirus). Disconcertingly, even after seven months from the first r
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