A sensitive and rapid bacterial antibiotic susceptibility test method by surface enhanced Raman spectroscopy
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BACTERIAL AND FUNGAL PATHOGENESIS - RESEARCH PAPER
A sensitive and rapid bacterial antibiotic susceptibility test method by surface enhanced Raman spectroscopy Shijie Fu 1 & Xiwen Wang 2,3 & Ting Wang 4 & Zhiping Li 5 & Deming Han 1 & Chunsheng Yu 6 & Cui Yang 6 & Han Qu 5 & Hang Chi 5 & Yutian Wang 5 & Song Li 1 & Baihui Tian 1 & Wenliang Li 7 & Zhiping Xia 5 Received: 4 August 2019 / Accepted: 17 April 2020 # Sociedade Brasileira de Microbiologia 2020
Abstract This study aims to develop a rapid bacterial antibiotic susceptibility test (AST) method by Bacteria-aptamer@AgNPs–surface enhanced Raman spectroscopy (SERS) and further evaluate the influence of different antibiotics on the Raman intensity of bacteria. The Raman intensity of Escherichia coli O157:H7 (E. coli O157:H7) and Staphylococcus aureus (S. aureus) in the presence of different concentrations of antibiotics in 2 h was detected by Bacteria-aptamer@AgNPs-SERS in this study. Our results found that the bacteria Raman signal peak at 735 cm−1 and the minimum inhibitory concentration (MIC) value was determined in 1 h according to Raman signals. In 2 h, the bacteria Raman signal growth at sub-MIC concentrations of four different kinds of antibiotics and the bacteria colony-forming unit (CFU) have similar enhancements. SERS utilizes special functions of rough metal surfaces and offers a huge enhancement of Raman intensities with reduced fluorescence backgrounds, which makes it an ultrasensitive tool of detection. This rapid AST method and the enhancement effect should be of value in search of new antibiotic drugs. Keywords SERS . Antibiotics . Antibiotic susceptibility . MIC
Introduction Bacterial pathogens are one of the main causes of human incidence. Historically, antibiotics have been highly effective for most bacterial pathogens [1]. However, the resistance of bacteria to broad-spectrum antibiotics is increasing, which has become a global health care issue [2]. Early and rapid determination of bacterial susceptibility to antibiotics has become a necessary condition for clinical drug research and development [3].
Therefore, the appropriate methods to assess antibiotic susceptibility has become of paramount importance in the research. The direct detection of biological sample signal by Raman spectrum is low. SERS technology can enhance the signal of biological sample, so as to improve the intensity of Raman spectrum. SERS technology uses the specificity of Raman scattering photons and the characteristics of rough nanometal particles to enhance the signal [4]. Raman scattering is an inelastic process in which the energy levels of the incident
Responsible Editor: Elizabeth Andrade Marques. * Deming Han [email protected]
3
Key Laboratory of Jinlin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun 130021, Jilin, People’s Republic of China
4
Department of the Gastrointestinal Surgery, The First Hospital of Jilin University, Changchun 130021, Jilin, People’s Republic of Chin
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