A sandwich electrochemiluminescent assay for determination of concanavalin A with triple signal amplification based on M
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ORIGINAL PAPER
A sandwich electrochemiluminescent assay for determination of concanavalin A with triple signal amplification based on MoS2NF@MWCNTs modified electrode and Zn-MOF encapsulated luminol Tingting Tang 1,2 & Fan Yang 1 & Lina Wang 1,2 & Chunxin Zhao 1 & Fei Nie 1,2
&
GuopingYang 1
Received: 21 March 2020 / Accepted: 2 August 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract An ultrasensitive sandwich electrochemiluminescence (ECL) biosensor was designed for determination of concanavalin A (ConA) through the specific carbohydrate-ConA interactions. Three-dimensional porous metal-organic framework (Zn-MOF) was synthesized, which loaded a large amount of luminescent reagents as luminol by encapsulating into its pores to form ZnMOF@luminol complex. Interestingly, Zn-MOF also acted as the coreactant accelerator in the luminol-H2O2 ECL system. This Zn-MOF@luminol complex was used as the signal probe to achieve a super strong and stable ECL signal. In addition, threedimensional hierarchical molybdenum disulfide nanoflower and multiwalled carbon nanotubes complex (MoS2NF@MWCNTs) with peroxidase-mimicking enzyme property were used as a substrate to modify the glassy carbon electrode to further enhance the ECL signal of luminol by promoting decomposition of H2O2 into reactive oxygen species (ROSs). In addition to the horseradish peroxidase (HRP) catalysis effect on the luminol ECL signal, a triple amplified ConA sandwich ECL sensor with high sensitivity sensor was constructed. The linear range for ConA detection was from 0.5 pg/mL to 100 ng/mL with a detection limit of 0.3 pg/mL (S/N = 3). The recovery test for ConA in human serum samples was performed with satisfactory results.
Keywords Electrochemiluminescence . Metal-organic framework . Signal amplification . Biosensor . Concanavalin A
Introduction
Tingting Tang and Fan Yang contributed equally to this work and should be considered co-first authors. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04472-8) contains supplementary material, which is available to authorized users. * Fei Nie [email protected] * GuopingYang [email protected] 1
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry (Ministry of Education), College of Chemistry & Materials Science, Northwest University, Xi’an 710069, People’s Republic of China
2
Shaanxi Provincial Key Laboratory of Electroanalytical Chemistry, Northwest University, Xi’an 710069, People’s Republic of China
Exogenous lectins are proteins derived from plants, animals, or microorganisms. They have the ability to bind to sugar residues in cell walls or membranes. The interaction between carbohydrates and specific proteins regulates many important physiological and pathological processes, such as bacterial and viral infections, cancer metastasis, inflammation, and immune surveillance [1, 2]. Concanavalin A (ConA) is one of the most commonly researched lectins, which binds specifically to carbohydrates and glycoproteins [
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