Aptamer-based photoelectrochemical assay for the determination of MCF-7
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Aptamer-based photoelectrochemical assay for the determination of MCF-7 Junjun Luo 1 & Dong Liang 1 & Xiaoqing Li 1 & Lei Deng 1 & Zaoxia Wang 1 & Minghui Yang 1 Received: 5 November 2019 / Accepted: 24 March 2020 # Springer-Verlag GmbH Austria, part of Springer Nature 2020
Abstract In this work, an aptamer-based photoelectrochemical (PEC) assay is reported for the determination of MCF-7 breast cancer cells using hexagonal carbon nitride tubes (HCNTs) as photoactive material. The aptamer immobilized on the HCNT surface can specifically bind with mucin 1 protein (MUC1) that is overexpressed on the surface of MCF-7 cell. Thus, the PEC assay has high specificity for the determination of MCF-7. The determination of MCF-7 is due to the binding of MCF-7 onto HCNT that suppressed the photocurrent intensity. The PEC assay displays good performances for MCF-7 determination with a linear range from 1 × 102 to 1 × 105 cell mL−1 and limit of detection down to 17 cells mL−1. Meanwhile, the PEC assay can distinguish MCF7 from normal cells in blood samples, which may have potential applications in cancer diagnostics and therapeutics. Keywords Photoelectrochemical assay . Hexagonal carbon nitride tube . MCF-7 . Aptamer
Introduction Circulating tumor cells (CTCs) are tumor cells that originate from primary tumor sites into peripheral blood [1–3]. CTCs can form metastasis in the resident organs when they pass through the circulatory system, which is responsible for 90% of the cancer-related deaths [4]. However, it is of great challenge for precise CTC determination due to its extremely rare amount [5–9]. Thus, developing ultrasensitive approaches for the determination of CTCs is of great importance. To date, various CTC determination methods have been reported based on fluorescence [10], microfluidics [11], quartz crystal microbalance (QCM) [12], differential pulse voltammetry (DPV) [13], photoelectrochemistry [14], photoacoustic method [15], and electrochemiluminescence (ECL) [16, 17]. Among these methods, photoelectrochemical (PEC) method is particularly attractive for bioanalysis [18, 19]. By Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00604-020-04239-1) contains supplementary material, which is available to authorized users. * Minghui Yang [email protected] 1
Hunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China
combining an electrochemical instrument and an irradiation source, PEC method has advantage of complete separation of the light excitation source with photocurrent signal, which is beneficial to reduce background signals and improve sensitivity [20, 21]. In comparison with conventional optical methods, the photoelectrochemical instrument is simple and portable [22]. However, PEC method has rarely been reported for the determination of CTCs [23, 24]. Therefore, it is highly meaningful to develop PEC method for CTC determinati
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