A Simple and Sensitive Nanocatalytic Fluorescence Method for the Determination of Folic Acid in Foods Using Fe 3 O 4 Nan
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A Simple and Sensitive Nanocatalytic Fluorescence Method for the Determination of Folic Acid in Foods Using Fe3O4 Nanoparticle-K2S2O8 System Huixiang Ouyang & Lisheng Wang & Jianniao Tian & Aihui Liang & Zhiliang Jiang
Received: 2 February 2012 / Accepted: 3 April 2012 / Published online: 26 April 2012 # Springer Science+Business Media, LLC 2012
Abstract In pH 2.72 HCl-NaAc buffer solution at room temperature and in the presence of a nanocatalyst of Fe3O4 nanoparticles, potassium persulfate rapidly oxidized folic acid (FA) to form a product with strong fluorescence at 446 nm. The analytical conditions were considered, and a simple, rapid, and accurate fluorescence method was proposed for the determination of 0.007–8.0 mg/L FA, with a detection limit of 2.0 ng/mL FA. This method was applied to the analysis of FA in a real sample with satisfactory results that agree with the HPLC results. Keywords Folic acid . Fe3O4 nanoparticle . Nanocatalytic fluorescence method
Introduction Folic acid (FA) (Stanger 2002), a water-soluble vitamin B, is consisted of a pterin moiety, p-aminobenzoic acid and glutamic acid. In 1941, it was purified from spinach leaves; thus, it was named folic acid. FA is a necessary vitamin to form the normal red blood cells; its deficiency could induce megaloblastic anemia. Moreover, FA plays an important auxiliary role in the biochemistry synthesis' methylation cycle as well as in the transmission link of “one-carbon units” of the DNA biosynthesis process. The deficiency of FA can also lead to H. Ouyang : L. Wang : J. Tian : A. Liang : Z. Jiang (*) Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection of the Ministry of Education, Guangxi Key Laboratory of Environmental Engineering and Protection and Assessment, Guangxi Normal University, Guilin 541004, China e-mail: [email protected]
failure of the synthesis of purine and thymine, which induced DNA defects (Stanger 2002), especially in the prepregnancy or pregnancy wherein the lack of FA would increase the risk of infant malformations. In recent years, it was reported that folic acid deficiency had also been implicated in cardiovascular disease, nosohemia, neurological disorders, and cancers (Rezk et al. 2003; Joshi et al. 2001). FA is widely present in animal and plant foods, especially in yeast, liver, and greenstuff. However, the FA which we can absorb from the natural food is rare because the natural folic acid can be oxidized easily by sunlight and high temperature. So, in order to prevent folic acid deficiency, we have added the artificial FA into some foods, especially infant food, or taken the FA tablets directly. Currently, the main methods for detecting FA include chemiluminescence (Nie et al. 2003; Zhang et al. 2008; Zhao et al. 2006), spectrophotometry (Andrisano et al. 2003; Rodríguez Flores et al. 2005; Dietmar 2001; Alaburda et al. 2008; María et al. 2004; Arcot et al. 2002), surface plasmon resonance (Caselunghe and Lindeberg 2000), fluorescence (Cruces Blanco et al. 1995), resonance scatt
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