A standardized method for rearing Rhizoglyphus robini (Astigmata: Acaridae)
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A standardized method for rearing Rhizoglyphus robini (Astigmata: Acaridae) Arash Ghalehgolabbehbahani1 · Margaret Skinner2 · Bruce L. Parker1 · Ali Razavi1 · Paul Reese1 · Agrin Davari1 Received: 28 July 2020 / Accepted: 16 September 2020 © Deutsche Phytomedizinische Gesellschaft 2020
Abstract Rhizoglyphus robini Claparede (Acari: Astigmata: Acaridae) is a mite infesting crop such as saffron corms, onions, garlic, leeks and lilies. Bulb mites were observed associated with diseased saffron corms in Vermont research sites, confirming their pest status in New England. To assess the efficacy of different IPM and biological controls a standard method to rear bulb mites in the laboratory was essential. This study focused on developing an efficient laboratory medium to rear R. robini. Bulb mites were extracted from infected saffron corms in Vermont (USDA plant-hardiness zone 5a [− 20° to − 15 °F]). A bulb mite culture was first established on potato dextrose agar (PDA) medium with an average moisture content of 67% at the Entomology Research Laboratory, University of Vermont. Bulb mite species identification and R. robini separation were done, and bulb mites were incubated in a dark chamber at 25 °C. While establishing the R. robini laboratory culture, contamination of microbial disease restricted populations. Experiments were conducted to determine the most suitable growth medium that supported bulb mite population and minimized microbial disease. Three treatments were tested: 1. antibiotics (chloramphenicol) + fungicide (dodine) + PDA, 2. antibiotics + PDA and 3. PDA only. The results showed significantly (P ≤ 0.05) larger populations of R. robini in Petri dishes containing PDA with antibiotics compared to other treatments. The greatest number of bulb mites was observed 14 to 20 days after introduction on the growth media. Keywords Bulb mites · Rhizoglyphus robini · Biological control · Potato dextrose agar · Laboratory culture
Introduction Bulb mites from the Acaridae family are pests of economically important crops with corms, bulbs and tubers (Diaz et al. 2000; Baker 2009). Rhizoglyphus robini Claparede * Arash Ghalehgolabbehbahani [email protected] Margaret Skinner [email protected] Bruce L. Parker [email protected] Ali Razavi [email protected] Paul Reese [email protected] Agrin Davari [email protected] 1
Entomology Research Laboratory, University of Vermont, 661 Spear Street, Burlington, VT 05405‑0105, USA
Plant and Soil Science Department, University of Vermont, 661 Spear St., Burlington, VT 05405, USA
2
(Acari: Astigmata: Acaridae) is a mite pest species that infests saffron corms (Rahimi et al. 2008), grains, onions, lilies, garlic and leeks (Ofek et al. 2014). R. robini reduces plant growth and flowering by eating bulb scales (Lesna 2000) and feeding on plant tissue infected by fungi such as Fusarium oxysporum (Ofek et al. 2014). The life cycle of R. robini is optimized at 25° C under experimental settings (Diaz et al. 2000; Baker 2009). Various management tactics
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